It is well known that 2-Deoxy-D-glucose (2-DG) blocks intracellular utilization of glucose and increases food intake. The aim of the present study was to determine whether administration of 2-DG alters gene expression of the orexigenic peptides, neuropeptide Y (NPY) and endogenous opioids, in the arcuate nucleus of the hypothalamus (ARC). Male Sprague-Dawley rats were injected peripherally (i.p.) with 2-DG (200 or 400 mg/kg body weight) and were sacrificed at 2 or 6 h post injection. Half of the animals were given ad libitum access to food whereas the other half of the animals were food- deprived. 2-DG increased food intake fourfold compared to saline injected animals, but did not affect NPY mRNA levels after 2 h. Messenger RNA levels of ProDynorphin (proDYN), but not pro-opiomelanocortin (POMC) nor proEnkephalin (proENK) were significantly decreased 2 h after 2-DG injection. Administration of 400 mg/kg of 2-DG increased mRNA levels of NPY in the arcuate nucleus after six h, but only in those animals not receiving food.
Bibliographical noteFunding Information:
This research was supported by the General Research Funds of the Veterans Administration Medical Center, the National Institute of Drug Abuse Grant DA-03999 and by the National Institute of Diabetes and Digestive Diseases Grant DK 50456.
Copyright 2007 Elsevier B.V., All rights reserved.
- 2-deoxy-D-glucose (2-DG)
- Neuropeptide Y (NPY)
- Pro- opiomelanocortin (POMC)
- ProDynorphin (proDYN)
- ProEnkephalin (proENK)