The effect of vibrational relaxation on turbulent motion is studied for compressible isotropic turbulence. The flow-field is initialized with a nonzero divergence velocity field and fluctuating density, temperature, and pressure. The effect of turbulent Mach number and temperature is observed to have a similar effect on the fluctuating quantities as that of altering the vibrational relaxation time. No significant difference in the decay of turbulent kinetic energy was observed when vibrational relaxation is included. The thermodynamic fluctuating quantities are shown to be damped by vibrational relaxation. This damping increases as the vibrational relaxation rate is increased. The primary energy exchange is observed to be between the translational and vibrational energy modes. Virtually no exchange between kinetic and internal energy modes is seen.