Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation

C. H. Hensel, R. B. Petersen, P. B. Hackett

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The 372-nucleotide leader sequence of Rous sarcoma virus RNA contains three conserved short open reading frames and other sequences responsible for a variety of life cycle functions. We have investigated several aspects of the leader RNA which may influence the translation of the major coding regions to which the leader is juxtaposed. We found that small perturbations of the leader length do not affect the binding and scanning of ribosomal subunits by more than about 10%, that the length and/or structure of the RSV RNA leader is near optimal for translation of the major coding regions of the viral RNA, that inclusion or deletion of open reading frames influences downstream initiation in a manner that is not strictly additive, and that reinitiation of translation at the gag gene is very efficient.

Original languageEnglish (US)
Pages (from-to)4986-4990
Number of pages5
JournalJournal of virology
Volume63
Issue number11
DOIs
StatePublished - 1989

Fingerprint Dive into the research topics of 'Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation'. Together they form a unique fingerprint.

Cite this