TY - JOUR
T1 - Effects of clofibrate and acetylsalicylic acid on hepatic carnitine palmitoyltransferase synthesis
AU - Brady, Paul S.
AU - Brady, Linda J.
PY - 1989/3/1
Y1 - 1989/3/1
N2 - Clofibrate and acetylsalicylic and have both been reported to increase carnitine palmitoyltransferase (CPT) activity when administered to rats. The purpose of the present study was to determine the mechanism of the increase in CPT activity. Rats (150-200 g) were fed one of the following: chow, chow with 0.5% clofibrate, or chow with 1% acetylsalicylic acid for 2 weeks. At the end of this time, hepatic CPT activity was increased 4-fold over control in the clofibrate group and 3.6-fold over control in the acetylsalicylic acid group. Immunoreactive protein increased 4.0- and 3.6-fold, respectively, over control. Transcription rates of hepatic nuclei were increased 2.8- and 1.9-fold over control in the clofibrate and acetylsalicylic add groups, and hepatic mRNA levels increased 2.8- and 2.0-fold respectively. These data indicate that increases in CPT activity caused by clofibrate and acetylsalicylic acid administration are due, at least in part, to increased CPT protein, resulting from increased transcription rate and levels of mRNA specific for CPT.
AB - Clofibrate and acetylsalicylic and have both been reported to increase carnitine palmitoyltransferase (CPT) activity when administered to rats. The purpose of the present study was to determine the mechanism of the increase in CPT activity. Rats (150-200 g) were fed one of the following: chow, chow with 0.5% clofibrate, or chow with 1% acetylsalicylic acid for 2 weeks. At the end of this time, hepatic CPT activity was increased 4-fold over control in the clofibrate group and 3.6-fold over control in the acetylsalicylic acid group. Immunoreactive protein increased 4.0- and 3.6-fold, respectively, over control. Transcription rates of hepatic nuclei were increased 2.8- and 1.9-fold over control in the clofibrate and acetylsalicylic add groups, and hepatic mRNA levels increased 2.8- and 2.0-fold respectively. These data indicate that increases in CPT activity caused by clofibrate and acetylsalicylic acid administration are due, at least in part, to increased CPT protein, resulting from increased transcription rate and levels of mRNA specific for CPT.
UR - http://www.scopus.com/inward/record.url?scp=0024554247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024554247&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(89)90235-9
DO - 10.1016/0006-2952(89)90235-9
M3 - Article
C2 - 2930581
AN - SCOPUS:0024554247
SN - 0006-2952
VL - 38
SP - 811
EP - 814
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 5
ER -