Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) and probiotics on immune responses and caecal counts in young laying hens

J. S. Lee, M. J. Kim, S. H. Park, S. B. Lee, T. Wang, U. S. Jung, J. Im, E. J. Kim, K. W. Lee, H. G. Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

This study was conducted to evaluate the effects of a combined mixture of phytogenic extracts (garlic and coriander) and probiotics on growth performance and immune responses in laying hens based on the results of in vitro studies to screen for immunomodulatory potency of each ingredient. Several parameters of immunomodulatory potency were estimated using lamina propria leucocytes (LPLs) isolated from rat intestinal mucosa tissue. Results show that the combined mixture enhanced LPLs proliferation, increased LPL-mediated cytotoxicity against YAC-1 tumour cells, and decreased lipopolysaccharide (LPS)-induced cytokine production including tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in LPLs. For in vivo study, laying hens (n = 50/each diet group) were fed with control diet, a diet containing antibiotics (0.01% per kg feed) or the combined mixture (0.02% per kg feed) for 21 days. The dietary combined mixture improved egg production (p < 0.05) but not growth performance and carcass traits. Interestingly, the patterns of suppressing plasma IFN-γ productions during inflammation by LPS injection and decreasing caecal E. coli counts in the combined mixture group were comparable to those in the antibiotics group. Taken together, our results suggested that the 0.02% of combined mixture of phytogenic extracts and probiotics as ingredients has potential immunomodulatory effects in laying hens.

Original languageEnglish (US)
Pages (from-to)e122-e132
JournalJournal of Animal Physiology and Animal Nutrition
Volume101
Issue number5
DOIs
StatePublished - Oct 2017

Bibliographical note

Funding Information:
This paper was supported by Konkuk University in 2013.

Publisher Copyright:
Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH

Keywords

  • caecal bacteria
  • coriander
  • garlic
  • laying hens
  • probiotics
  • rat lamina propria leucocytes

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