Effects of ELF capacitively coupled weak electric fields on metabolism of 6B1 cells

Xing Wang, Aihua Zhou, Man Liu, Haiying Yu, Lijun Pang, Mingyao Zhu, Leiguang Wang, Hermann Berg

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we adopted several methods of MTT colorimetry, DAPI fluorimetry and ELISA to study the effects of extremely low frequency (ELF) capacitively coupled electric fields (EFs) on the metabolism of 6B1 cells. The result shows that 50 mV cm-1 ELF EF (10-100 Hz) has no significant effect on proliferation, DNA synthesis and activity of succinate dehydrogenase of 6B1 cells, indicating that the effect of ELF (10-100 Hz) EF on the metabolism of 6B1 cells is not obvious. However, 50 mV cm-1, 50 Hz EF significantly promotes the HBs-Ab (Hepatitis B surface antibody) secretion of 6B1 cells, implying that under this situation, EF has some distinctive effect on the outerface of 6B1 cell membrane. Copyright (C) 1999 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalBioelectrochemistry and Bioenergetics
Volume48
Issue number2
DOIs
StatePublished - May 1999
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (Project 39500033). We thank Prof. Hemu Wang and Prof. Yuwa Lu for their energetic support: supply 6B1 cells and technical help on cell culture and measurement.

Keywords

  • 6B1 cells
  • HBs-Ab

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