In this study, we adopted several methods of MTT colorimetry, DAPI fluorimetry and ELISA to study the effects of extremely low frequency (ELF) capacitively coupled electric fields (EFs) on the metabolism of 6B1 cells. The result shows that 50 mV cm-1 ELF EF (10-100 Hz) has no significant effect on proliferation, DNA synthesis and activity of succinate dehydrogenase of 6B1 cells, indicating that the effect of ELF (10-100 Hz) EF on the metabolism of 6B1 cells is not obvious. However, 50 mV cm-1, 50 Hz EF significantly promotes the HBs-Ab (Hepatitis B surface antibody) secretion of 6B1 cells, implying that under this situation, EF has some distinctive effect on the outerface of 6B1 cell membrane. Copyright (C) 1999 Elsevier Science S.A.
Bibliographical noteFunding Information:
This work was supported by the National Natural Science Foundation of China (Project 39500033). We thank Prof. Hemu Wang and Prof. Yuwa Lu for their energetic support: supply 6B1 cells and technical help on cell culture and measurement.
- 6B1 cells