Effects of epidermal growth factor and transforming growth factor-β1 on rat heart endothelial cell anchorage-dependent and -independent growth

Daniel L. Mooradian, Clement A. Diglio

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11 Scopus citations

Abstract

This report describes the effects of epidermal growth factor (EGF) and transforming growth factor-β1 (TGF-β1) on the anchorage-dependent and -independent growth of rat heart endothelial cells (RHE-1A). When RHE-1A cells were grown in monolayer culture with medium containing 10% fetal bovine serum (FBS) supplemented with epidermal growth factor (0.1-100 ng/ ml), growth was stimulated fivefold when compared to that of cells grown in medium containing 10% FBS alone. The stimulatory effect of EGF on RHE-1A cell monolayer growth was dose-dependent and half-maximal at 5 ng/ml. The addition of TGF-β1 in the range 0.1-10 ng/ml had no effect on RHE-1A cell monolayer growth when added to medium containing 10% FBS alone or 10% FBS supplemented with EGF (50 ng/ml). RHE-1A cells failed to grow under anchorage-independent conditions in 0.3% agar medium containing 10% FBS. In the presence of EGF, however, colony formation increased dramatically. The stimulatory effect of EGF was dose-dependent in the range 0.1-100 ng/ml and was half-maximal at 5 ng/ml. In contrast to its effects under anchorage-dependent conditions, TGF-β1 (0.1-10 ng/ml) antagonized the stimulatory effects of EGF on RHE-1A cell anchorage-independent growth. The inhibitory effect of TGF-β1 was dose-dependent and half-maximal at 0.1 ng/ml. EGF-induced RHE-1A soft agar colonies were isolated and reinitiated in monolayer culture. They retained the cobblestone morphology and contact-inhibition characteristic of normal vascular endothelial cells. Each of the clones continued to express Factor VIII antigen. These findings suggest that TGF-β may influence not only endothelial cell proliferation but also anchorage dependence. These effects may in turn be of relevance to endothelial cell growth and angiogenesis in vivo.

Original languageEnglish (US)
Pages (from-to)122-129
Number of pages8
JournalExperimental Cell Research
Volume186
Issue number1
DOIs
StatePublished - Jan 1990

Bibliographical note

Funding Information:
* This work is part of a doctoral thesis by Daniel L. Mooradian to fulfill the requirements of the Graduate School of Wayne State Uni-versity. This work was supported in part by U.S. Public Health Service Grant HL-23603 and by a postdoctoral fellowship from the Minnesota Affiliate of the American Heart Association.

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