TY - JOUR
T1 - Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues
AU - Nakao, Chitose
AU - Ookawara, Tomomi
AU - Kizaki, Takako
AU - Oh-Ishi, Shuji
AU - Miyazaki, Hiromi
AU - Haga, Shuhkoh
AU - Sato, Yuzo
AU - Ji, Li Li
AU - Ohno, Hideki
PY - 2000/2
Y1 - 2000/2
N2 - The purpose of the present study was to investigate the effects of swimming training on the changes in three superoxide dismutase (SOD) isoenzymes in mice. The trained mice underwent a 6-wk swimming program (1 h/day, 5 days/wk) in water at 35-36°C. Immunoreactive extracellular SOD (EC- SOD), copper- and zinc-containing SOD (CuZn-SOD), and manganese-containing SOD (Mn-SOD) contents and their mRNA abundance were determined in serum, heart, lung, liver, kidney, and gastrocnemius muscle. EC-SOD content in liver and kidney was significantly increased with training. After training, CuZn- SOD content rose significantly only in kidney but decreased significantly in heart, lung, and liver. Mn-SOD content showed a significant increase in lung, kidney, and skeletal muscle but a significant decrease in liver. In most tissues, however, the changes in SOD isoenzyme contents were not concomitant with those in their mRNA levels. The results obtained thus suggest that, except for kidney, the responses in mouse tissues of three SOD isoenzymes (protein levels and mRNA abundance) to swimming training are different and that kidney may be one of the most sensitive organs to adapt to oxidative stress during physical training, although the mechanism remains vague.
AB - The purpose of the present study was to investigate the effects of swimming training on the changes in three superoxide dismutase (SOD) isoenzymes in mice. The trained mice underwent a 6-wk swimming program (1 h/day, 5 days/wk) in water at 35-36°C. Immunoreactive extracellular SOD (EC- SOD), copper- and zinc-containing SOD (CuZn-SOD), and manganese-containing SOD (Mn-SOD) contents and their mRNA abundance were determined in serum, heart, lung, liver, kidney, and gastrocnemius muscle. EC-SOD content in liver and kidney was significantly increased with training. After training, CuZn- SOD content rose significantly only in kidney but decreased significantly in heart, lung, and liver. Mn-SOD content showed a significant increase in lung, kidney, and skeletal muscle but a significant decrease in liver. In most tissues, however, the changes in SOD isoenzyme contents were not concomitant with those in their mRNA levels. The results obtained thus suggest that, except for kidney, the responses in mouse tissues of three SOD isoenzymes (protein levels and mRNA abundance) to swimming training are different and that kidney may be one of the most sensitive organs to adapt to oxidative stress during physical training, although the mechanism remains vague.
KW - Affinity for heparin
KW - Extracellular superoxide dismutase
KW - mRNA
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U2 - 10.1152/jappl.2000.88.2.649
DO - 10.1152/jappl.2000.88.2.649
M3 - Article
C2 - 10658033
AN - SCOPUS:0033974490
SN - 8750-7587
VL - 88
SP - 649
EP - 654
JO - Journal of applied physiology
JF - Journal of applied physiology
IS - 2
ER -