This study investigated the effect of electrolyzed water on pathogenic bacteria cell suspensions. Specifically, we evaluated the efficacy of strong and weak acidic electrolyzed waters (SACEW, WACEW) and strong and weak alkaline electrolyzed waters (SALEW, WALEW) on Vibrio parahaemolyticus, Listeria monocytogenes, Aeromonas hydrophila, Campylobacter jejuni, and Escherichia coli O104:H4 in suspensions of (10 7 -10 9 CFU/mL) in 1% NaCl. SACEW and WACEW were applied at available chlorine concentrations (ACC) of 20 and 10mg/mL, pH 3.1 and 3.55 and oxidation-reduction potentials (ORP) of 1150 and 950mV, respectively. Results show that no viable cells were recovered for V.parahaemolyticus, L.monocytogenes, A.hydrophila, C.jejuni within 2min at 20°C. However, E.coli O104:H4 was significantly more resistant to ALEW compared to ACEW. Results also show that the bactericidal activity of SACEW (20mg/mL ACC) was more effective than WACEW (10mg/mL ACC) in terms of inactivating E.coli O104:H4. Alkaline-electrolyzed waters were found to reduce cell numbers by 1-3 log (P<0.05). However, alkaline electrolyzed water was less effective (P<0.05) than acidic electrolyzed treatment.
Bibliographical noteFunding Information:
This study was funded by USDA-NIFA 2011-68003-20096 , the Agricultural Research Center at Washington State University and the University of Jordan with the grant number of 1409 .
© 2015 Elsevier Ltd.
- Acidic electrolyzed water
- Alkaline electrolyzed water
- Cell suspensions
- Pathogenic bacteria