Electron microscopic and 31-P NMR studies of ischemic injured rat livers during preservation and reflow

J. Ellermann, B. Gewiese, H. David, D. Stiller, M. Plötz, K. J. Wolf, M. Lüning

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Ischemic injury induced during preservation and reperfusion contributes to post-operative failure in liver transplantation. Hepatic injury and recovery from preservation was studied in an isolated rat liver model reperfused with oxygenated erythrocytes. In order to correlate morphological and functional findings, 31-P nuclear magnetic resonance spectroscopy and electron microscopy were used to investigate metabolic and ultrastructural changes during 6 hours of reperfusion. Following cold preservation, EM's showed a primary sinusoidal cell injury, whereas the hepatocytes were well maintained. During reperfusion, hepatocytes displayed further damage. The simultaneous presence of vacuolarly degenerated mitochondria and mitochondria of increased activity was noted. 31-P NMP spectra demonstrated initially a partial ATP-recovery. The maximum level of 60% of the control ATP-value could not be further increased. EM and 31-P NMR indicate that the progressive injury to the liver is due to microcirculatory malfunction induced by an endothelial cell damage, followed by injured hepatocytes themselves, and the consequent intracellular energy crisis that is produced.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalExperimental and Toxicologic Pathology
Volume44
Issue number5
DOIs
StatePublished - Jan 1 1992

Keywords

  • 31-P nuclear magnetic resonance spectroscopy
  • Endothelial cell damage, liver
  • Hepatocyte, ischemic injury
  • Ischemic injury, liver
  • Liver, endothelial cell damage
  • Liver, ischemic injury
  • Liver, microcirculatory malfunction
  • Liver, post-operative failure
  • Liver, reperfusion
  • Liver, sinusoidal cell injury
  • Microcirculatory malfunction, liver
  • Reperfusion, Liver
  • Sinusoidal cell injury, liver

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