Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins

Rebecca J. Moen, Jennifer C. Klein, David D. Thomas

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalExercise and sport sciences reviews
Volume42
Issue number1
DOIs
StatePublished - Jan 2014

Keywords

  • ROS
  • actin
  • aging
  • methionine
  • muscle
  • myosin
  • reactive oxygen species

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