Abstract
We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.
Original language | English (US) |
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Pages (from-to) | 30-36 |
Number of pages | 7 |
Journal | Exercise and sport sciences reviews |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2014 |
Keywords
- ROS
- actin
- aging
- methionine
- muscle
- myosin
- reactive oxygen species