Electrospray lonization Mass Spectral Characterization of Transient Iron Species of Bioinorganic Relevance

Jinheung Kim; Yanhong Dong; Edmund Larka; Larry Que

Electrospray lonization Mass Spectral Characterization of Transient Iron Species of Bioinorganic Relevance

Jinheung Kim, Yanhong Dong, Edmund Larka, Larry Que

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Abstract

Strategies have been developed to obtain electrospray ionization mass spectral data on short-lived intermediates derived from the reactions of non-heme iron complexes with peroxides. The molecular composition of a transient green intermediate generated from [Fe₂O(5-Me₃-TPA)₂(OH)(H₂O)](ClO ₄)₃ with H₂O₂ in CH₃CN at -40 °C was determined by introducing the solution via a precooled syringe to the inlet of the mass spectrometer. The observation of prominent ion clusters in both positive and negative mass regions, together with isotope distribution patterns characteristic of the number of associated ClO₄ ions, allows its formulation as [Fea(O)₂(5-Me₃-TPA)₂]-(ClO₄) ₃. The molecular composition of a transient blue species derived from the reaction of [Fe₂O(TPA)₂-(H₂O)(ClO₄)](ClO ₄)₃ with excess benzyl alcohol and 'BuOOH at -40 °C was also determined. Due to its limited stability even at -40 °C, the blue intermediate was generated in a cooled mixing tee from its precursor components and directly introduced into the mass spectrometer. Its formulation as {[Fe(TPA)(OO^tBu)(ROH)](ClO₄)}⁺ (R = H or CH₂Ph) is based on the masses observed, isotope distribution patterns, the observation of expected shifts in the mass values by appropriate substitutions, and tandem mass spectral data. These transient species relate to possible intermediates of non-heme iron enzymes.

Original language	English (US)
Pages (from-to)	2369-2372
Number of pages	4
Journal	Inorganic Chemistry
Volume	35
Issue number	8
State	Published - Dec 1 1996

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abstract = "Strategies have been developed to obtain electrospray ionization mass spectral data on short-lived intermediates derived from the reactions of non-heme iron complexes with peroxides. The molecular composition of a transient green intermediate generated from [Fe2O(5-Me3-TPA)2(OH)(H2O)](ClO 4)3 with H2O2 in CH3CN at -40 °C was determined by introducing the solution via a precooled syringe to the inlet of the mass spectrometer. The observation of prominent ion clusters in both positive and negative mass regions, together with isotope distribution patterns characteristic of the number of associated ClO4 ions, allows its formulation as [Fea(O)2(5-Me3-TPA)2]-(ClO4) 3. The molecular composition of a transient blue species derived from the reaction of [Fe2O(TPA)2-(H2O)(ClO4)](ClO 4)3 with excess benzyl alcohol and 'BuOOH at -40 °C was also determined. Due to its limited stability even at -40 °C, the blue intermediate was generated in a cooled mixing tee from its precursor components and directly introduced into the mass spectrometer. Its formulation as {[Fe(TPA)(OOtBu)(ROH)](ClO4)}+ (R = H or CH2Ph) is based on the masses observed, isotope distribution patterns, the observation of expected shifts in the mass values by appropriate substitutions, and tandem mass spectral data. These transient species relate to possible intermediates of non-heme iron enzymes.",

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AB - Strategies have been developed to obtain electrospray ionization mass spectral data on short-lived intermediates derived from the reactions of non-heme iron complexes with peroxides. The molecular composition of a transient green intermediate generated from [Fe2O(5-Me3-TPA)2(OH)(H2O)](ClO 4)3 with H2O2 in CH3CN at -40 °C was determined by introducing the solution via a precooled syringe to the inlet of the mass spectrometer. The observation of prominent ion clusters in both positive and negative mass regions, together with isotope distribution patterns characteristic of the number of associated ClO4 ions, allows its formulation as [Fea(O)2(5-Me3-TPA)2]-(ClO4) 3. The molecular composition of a transient blue species derived from the reaction of [Fe2O(TPA)2-(H2O)(ClO4)](ClO 4)3 with excess benzyl alcohol and 'BuOOH at -40 °C was also determined. Due to its limited stability even at -40 °C, the blue intermediate was generated in a cooled mixing tee from its precursor components and directly introduced into the mass spectrometer. Its formulation as {[Fe(TPA)(OOtBu)(ROH)](ClO4)}+ (R = H or CH2Ph) is based on the masses observed, isotope distribution patterns, the observation of expected shifts in the mass values by appropriate substitutions, and tandem mass spectral data. These transient species relate to possible intermediates of non-heme iron enzymes.

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Electrospray lonization Mass Spectral Characterization of Transient Iron Species of Bioinorganic Relevance

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