TY - GEN
T1 - Electrospun poly (styrene-co-maleic anhydride) nanofibers for β-D-galactosidase immobilization and enzymatic transgalactosylation
AU - Li, Pengju
AU - Zhang, Yufei
AU - Zhang, Songping
AU - Wang, Ping
AU - Wang, Min
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Poly(styrene-co-maleic anhydride) nanofibers with a uniform diameter of about 400 nm were prepared by eletrospinning a solution containing 50 % (w/v) polymer and 1% (w/v) LiCl, 25 cm gap between jection needle tip and collector, a flow rate of 200 μL/h, and an applied voltage of 24 kV. β-D- galactosidase was immobilized to the electrospun nanofibers and subsequently used for synthesizing hexyl-galactoside, an important nonionic surfactants, by catalyzing the transgalactosylation reaction between hexanol and lactose. Co-electrospun LiCl was found leading to a significant enhancement in the enzyme loading and activity of the immobilized β-D-galactosidase compared to nanofiber without LiCl. After 7 reusing cycles at 37°C, the immobilized enzyme retained about 33% of its original activity.
AB - Poly(styrene-co-maleic anhydride) nanofibers with a uniform diameter of about 400 nm were prepared by eletrospinning a solution containing 50 % (w/v) polymer and 1% (w/v) LiCl, 25 cm gap between jection needle tip and collector, a flow rate of 200 μL/h, and an applied voltage of 24 kV. β-D- galactosidase was immobilized to the electrospun nanofibers and subsequently used for synthesizing hexyl-galactoside, an important nonionic surfactants, by catalyzing the transgalactosylation reaction between hexanol and lactose. Co-electrospun LiCl was found leading to a significant enhancement in the enzyme loading and activity of the immobilized β-D-galactosidase compared to nanofiber without LiCl. After 7 reusing cycles at 37°C, the immobilized enzyme retained about 33% of its original activity.
KW - Electrospinning
KW - Immobilization
KW - Nanofibers
KW - Transgalactosylation
KW - β-D-Galactosidase
UR - http://www.scopus.com/inward/record.url?scp=83755183472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=83755183472&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.396-398.1394
DO - 10.4028/www.scientific.net/AMR.396-398.1394
M3 - Conference contribution
AN - SCOPUS:83755183472
SN - 9783037853085
T3 - Advanced Materials Research
SP - 1394
EP - 1397
BT - Advances in Chemical Engineering
T2 - 2011 International Conference on Chemical, Material and Metallurgical Engineering, ICCMME 2011
Y2 - 23 December 2011 through 25 December 2011
ER -