@inbook{bc02abd92e5d4f338d61385eb2c8a983,
title = "Embryological and genetic manipulation of chick development",
abstract = "The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.",
keywords = "Chick, Electroporation, Embryo culture, Morpholino oligonucleotide",
author = "Gammill, {Laura S} and Bridget Jacques-Fricke and Julaine Roffers-Agarwal",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
doi = "10.1007/978-1-4939-9009-2_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "75--97",
booktitle = "Methods in Molecular Biology",
}