Emergence of a novel Salmonella enterica serotype reading clonal group is linked to its expansion in commercial Turkey production, resulting in unanticipated human illness in North America

Elizabeth A. Miller, Ehud Elnekave, Cristian Flores-Figueroa, Abigail Johnson, Ashley Kearney, Jeannette Munoz-Aguayo, Kaitlin A. Tagg, Lorelee Tschetter, Bonnie P. Weber, Celine A. Nadon, Dave Boxrud, Randall S. Singer, Jason P. Folster, Timothy J. Johnson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Two separate human outbreaks of Salmonella enterica serotype Reading occurred between 2017 and 2019 in the United States and Canada, and both outbreaks were linked to the consumption of raw turkey products. In this study, a comprehensive genomic investigation was conducted to reconstruct the evolutionary history of S. Reading from turkeys and to determine the genomic context of outbreaks involving this infrequently isolated Salmonella serotype. A total of 988 isolates of U.S. origin were examined using whole-genome-based approaches, including current and historical isolates from humans, meat, and live food animals. Broadly, isolates clustered into three major clades, with one apparently highly adapted turkey clade. Within the turkey clade, isolates clustered into three subclades, including an "emergent" clade that contained only isolates dated 2016 or later, with many of the isolates from these outbreaks. Genomic differences were identified between emergent and other turkey subclades, suggesting that the apparent success of currently circulating subclades is, in part, attributable to plasmid acquisitions conferring antimicrobial resistance, gain of phage-like sequences with cargo virulence factors, and mutations in systems that may be involved in beta-glucuronidase activity and resistance towards colicins. U.S. and Canadian outbreak isolates were found interspersed throughout the emergent subclade and the other circulating subclade. The emergence of a novel S. Reading turkey subclade, coinciding temporally with expansion in commercial turkey production and with U.S. and Canadian human outbreaks, indicates that emergent strains with higher potential for niche success were likely vertically transferred and rapidly disseminated from a common source.

Original languageEnglish (US)
Article numbere00056-20
JournalmSphere
Volume5
Issue number2
DOIs
StatePublished - Mar 1 2020

Bibliographical note

Funding Information:
We thank the turkey producers of the United States for their willingness to collaborate in this study. Isolates and data for outbreak cases in Canada were provided courtesy of the PulseNet Canada Steering Committee and members of the Canadian Public Health Laboratory Network. Bioinformatics was supported using tools available from the Minnesota Supercomputing Institute. Sequencing reagents for this study were donated by the Mid-Central Research and Outreach Center, Willmar, MN, USA.

Keywords

  • Clone
  • Genomic
  • Human
  • Outbreak
  • Poultry
  • Salmonella
  • Turkey

PubMed: MeSH publication types

  • Journal Article

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