Abstract
A new chiral stationary phase based on continuous bed (CB) technology using L-prolinamide as a chiral selector was prepared. Its ability for enantioseparation of amino acids and a-hydroxy acids by ligand-exchange CEC was compared with that of a CB containing L-4-hydroxyproline as chiral selector. Preparation was done by a one-step in situ copolyermerization procedure using methacrylamide as monomer, vinylsulfonic acid as charge providing agent and piperazine diacrylamide as crosslinker. L-Prolinamide showed marked enantioselective properties for the separation of amino acids and α-hydroxy acids. Furthermore, we show the possibility of simultaneous enantioseparation of amino acids and α-hydroxy acids using the CB with L-4-hydroxyproline as chiral selector.
Original language | English (US) |
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Pages (from-to) | 1517-1520 |
Number of pages | 4 |
Journal | ELECTROPHORESIS |
Volume | 31 |
Issue number | 9 |
DOIs | |
State | Published - May 2010 |
Externally published | Yes |
Keywords
- Capillary electrochromatography
- Chiral ligand exchange
- L-4-hydroxyproline
- L-prolinamide
- Monolithic phase