Energy metabolism couples hepatocyte integrin-linked kinase to liver glucoregulation and postabsorptive responses of mice in an age-dependent manner

Elijah Trefts, Curtis C. Hughey, Louise Lantier, Dan S. Lark, Kelli L. Boyd, Ambra Pozzi, Roy Zent, David H. Wasserman

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Integrin-linked kinase (ILK) is a critical intracellular signaling node for integrin receptors. Its role in liver development is complex, as ILK deletion at E10.5 (before hepatocyte differentiation) results in biochemical and morphological differences that resolve as mice age. Nevertheless, mice with ILK depleted specifically in hepatocytes are protected from the hepatic insulin resistance during obesity. Despite the potential importance of hepatocyte ILK to metabolic health, it is unknown how ILK controls hepatic metabolism or glucoregulation. The present study tested the role of ILK in hepatic metabolism and glucoregulation by deleting it specifically in hepatocytes, using a cre-lox system that begins expression at E15.5 (after initiation of hepatocyte differentiation). These mice develop the most severe morphological and glucoregulatory abnormalities at 6 wk, but these gradually resolve with age. After identifying when the deletion of ILK caused a severe metabolic phenotype, in depth studies were performed at this time point to define the metabolic programs that coordinate control of glucoregulation that are regulated by ILK. We show that 6-wk-old ILK-deficient mice have higher glucose tolerance and decreased net glycogen synthesis. Additionally, ILK was shown to be necessary for transcription of mito-chondrial-related genes, oxidative metabolism, and maintenance of cellular energy status. Thus, ILK is required for maintaining hepatic transcriptional and metabolic programs that sustain oxidative metabolism, which are required for hepatic maintenance of glucose homeostasis.

Original languageEnglish (US)
Pages (from-to)E1118-E1135
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume316
Issue number6
DOIs
StatePublished - 2019
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by funding from the National Institutes of Health (Molecular Endocrinology Training Program, DK-007563, and an individual NRSA fellowship, DK-112553, to E. Trefts; DK-069221 and DK-083187 to R. Zent; and CA-162433 and DK-095761 to A. Pozzi; as well as DK-050277 and DK-054902 to D. H. Wasserman). This work was also made possible by Veterans Affairs Merit Reviews 1I01BX002025-01 to A. Pozzi and 1I01BX002196-01 to R. Zent. This work was also supported by The Diabetes Research and Training Center (DK-20593).

Publisher Copyright:
© 2019, American Physiological Society. All rights reserved.

Keywords

  • Glucose homeostasis
  • Glycogen metabolism
  • Hepatocyte signaling
  • In vivo physiology
  • Integrin signaling
  • Liver metabolism

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