Engineering cooperativity in biomotor-protein assemblies

Michael R. Diehl, Kechun Zhang, Heun Jin Lee, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

A biosynthetic approach was developed to control and probe cooperativity in multiunit biomotor assemblies by linking molecular motors to artificial protein scaffolds. This approach provides precise control over spatial and elastic coupling between motors. Cooperative interactions between monomeric kinesin-1 motors attached to protein scaffolds enhance hydrolysis activity and microtubule gliding velocity. However, these interactions are not influenced by changes in the elastic properties of the scaffold, distinguishing multimotor transport from that powered by unorganized monomeric motors. These results highlight the role of supramolecular architecture in determining mechanisms of collective transport.

Original languageEnglish (US)
Pages (from-to)1468-1471
Number of pages4
JournalScience
Volume311
Issue number5766
DOIs
StatePublished - Mar 10 2006

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