Engineering reversible cell-cell interactions with chemical biology

Clifford M. Csizmar, Carston R. Wagner

Research output: Chapter in Book/Report/Conference proceedingChapter


Techniques to direct cell-cell interactions have advanced our understanding of fundamental biology and opened new avenues in tissue engineering, regenerative medicine, and immunotherapy. This is often achieved by introducing new targeting ligands to the cell membrane, which can be accomplished through both genetic and nongenetic approaches. While both offer advantages, nongenetic modifications tend to be faster to produce, innocuous to the modified cell, and potentially reversible. This chapter will outline nongenetic methods that have been used to control intercellular interactions—namely hydrophobic insertion, chemical modification, liposome fusion, metabolic engineering, and enzymatic remodeling—and provide protocols that can serve as a starting point for future applications.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsDavid M. Chenoweth
PublisherAcademic Press Inc.
Number of pages24
ISBN (Print)9780128201459
StatePublished - 2020

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Bibliographical note

Funding Information:
The authors gratefully acknowledge support from the National Institutes of Health R21 CA185627 (C.R.W.), T32 GM008244 (C.M.C.), and the University of Minnesota Foundation.

Publisher Copyright:
© 2020 Elsevier Inc.


  • Cellular immunotherapy
  • Hydrophobic insertion
  • Intercellular interactions
  • Liposome fusion
  • Membrane engineering
  • Metabolic engineering
  • Regenerative medicine
  • Tissue engineering


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