TY - JOUR
T1 - Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction
AU - Jones, G. F.
AU - Ward, G. E.
AU - Murtaugh, M. P.
AU - Lin, G.
AU - Gebhart, C. J.
PY - 1993
Y1 - 1993
N2 - A sensitive assay based on amplification of a 319-bp DNA fragment of the intracellular bacterium of swine proliferative enteritis was developed for the detection of the organism in the feces of swine. A vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published for the intracellular bacterium, which was formerly known as a Campylobacter- like organism (C. J. Gebhart, S. M. Barnes, S. McOrist, G. F. Lin, and G. H. K. Larson, Int. J. Syst. Bacteriol. 43:533-538, 1993). As few as 101 IS- intracellularis organisms purified from intestinal mucosa, or 103 IS- intracellularis per g of feces, were detected. No amplification product was produced from a polymerase chain reaction performed on DNA extracted from the feces of healthy pigs. A 319-bp DNA fragment specific for IS-intracellularis was produced on amplification of DNA from the feces of pigs with experimental and naturally occurring proliferative enteritis.
AB - A sensitive assay based on amplification of a 319-bp DNA fragment of the intracellular bacterium of swine proliferative enteritis was developed for the detection of the organism in the feces of swine. A vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published for the intracellular bacterium, which was formerly known as a Campylobacter- like organism (C. J. Gebhart, S. M. Barnes, S. McOrist, G. F. Lin, and G. H. K. Larson, Int. J. Syst. Bacteriol. 43:533-538, 1993). As few as 101 IS- intracellularis organisms purified from intestinal mucosa, or 103 IS- intracellularis per g of feces, were detected. No amplification product was produced from a polymerase chain reaction performed on DNA extracted from the feces of healthy pigs. A 319-bp DNA fragment specific for IS-intracellularis was produced on amplification of DNA from the feces of pigs with experimental and naturally occurring proliferative enteritis.
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U2 - 10.1128/jcm.31.10.2611-2615.1993
DO - 10.1128/jcm.31.10.2611-2615.1993
M3 - Article
C2 - 8253956
AN - SCOPUS:0027504247
SN - 0095-1137
VL - 31
SP - 2611
EP - 2615
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 10
ER -