Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate

Hwan D. Kim; Jiyong Kim; Rachel H. Koh; Jimin Shim; Jong Chan Lee; Tae Il Kim; Nathaniel S. Hwang

doi:10.1021/acsbiomaterials.7b00299

Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate

Hwan D. Kim, Jiyong Kim, Rachel H. Koh, Jimin Shim, Jong Chan Lee, Tae Il Kim, Nathaniel S. Hwang

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.

Original language	English (US)
Pages (from-to)	2470-2479
Number of pages	10
Journal	ACS Biomaterials Science and Engineering
Volume	3
Issue number	10
DOIs	https://doi.org/10.1021/acsbiomaterials.7b00299
State	Published - Oct 9 2017

Bibliographical note

Funding Information:
Financial support for this work was provided from Seoul National University Dental Hospital Research Fund (01-2015-0006) and by the National Research Foundation of Korea (KNRF) grant funded by Ministry of Education, Science and Technology (NRF-2016R1E1A1A01943393). It is also supported by Global Ph.D. Fellowship Program from the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015H1A2A1029321).

Publisher Copyright:
© 2017 American Chemical Society.

Keywords

bone tissue engineering
graphene oxide incorporation
mesenchymal stem cells
osteogenesis
polyethylene glycol based cryogel

Access

10.1021/acsbiomaterials.7b00299

OpenUrl availability

Full text

Cite this

@article{70634ce0e166414399e751c5f22bee87,

title = "Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate",

abstract = "Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.",

keywords = "bone tissue engineering, graphene oxide incorporation, mesenchymal stem cells, osteogenesis, polyethylene glycol based cryogel",

author = "Kim, {Hwan D.} and Jiyong Kim and Koh, {Rachel H.} and Jimin Shim and Lee, {Jong Chan} and Kim, {Tae Il} and Hwang, {Nathaniel S.}",

note = "Funding Information: Financial support for this work was provided from Seoul National University Dental Hospital Research Fund (01-2015-0006) and by the National Research Foundation of Korea (KNRF) grant funded by Ministry of Education, Science and Technology (NRF-2016R1E1A1A01943393). It is also supported by Global Ph.D. Fellowship Program from the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015H1A2A1029321). Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = oct,

day = "9",

doi = "10.1021/acsbiomaterials.7b00299",

language = "English (US)",

volume = "3",

pages = "2470--2479",

journal = "ACS Biomaterials Science and Engineering",

issn = "2373-9878",

publisher = "American Chemical Society",

number = "10",

}

TY - JOUR

T1 - Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate

AU - Kim, Hwan D.

AU - Kim, Jiyong

AU - Koh, Rachel H.

AU - Shim, Jimin

AU - Lee, Jong Chan

AU - Kim, Tae Il

AU - Hwang, Nathaniel S.

N1 - Funding Information: Financial support for this work was provided from Seoul National University Dental Hospital Research Fund (01-2015-0006) and by the National Research Foundation of Korea (KNRF) grant funded by Ministry of Education, Science and Technology (NRF-2016R1E1A1A01943393). It is also supported by Global Ph.D. Fellowship Program from the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015H1A2A1029321). Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/10/9

Y1 - 2017/10/9

N2 - Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.

AB - Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.

KW - bone tissue engineering

KW - graphene oxide incorporation

KW - mesenchymal stem cells

KW - osteogenesis

KW - polyethylene glycol based cryogel

UR - http://www.scopus.com/inward/record.url?scp=85030830446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030830446&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.7b00299

DO - 10.1021/acsbiomaterials.7b00299

M3 - Article

AN - SCOPUS:85030830446

SN - 2373-9878

VL - 3

SP - 2470

EP - 2479

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

IS - 10

ER -

Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this