Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation

Mary G. Garry, Kenneth M. Hargreaves

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalBrain Research
Volume582
Issue number1
DOIs
StatePublished - Jun 5 1992

Bibliographical note

Funding Information:
The authors wish to thank Dr. Michael Vasko for his generous assistance with developing the superfusion technique. This research was supported in part by NRSA Fellowship F32-DEO5606 (M.G.G.) 3 Fitzgerald, M., Capsaicin sensitive neurons. A review, Pain, 15 (1983) 145-160. 4 Hargreaves, K., Dubner, R., Brown, E, Flores, C. and Joris, J., A new and sensitive method for measuring thermal nocicep-tion in cutaneous hyperalgesia, Pain, 32 (1988) 77-78. 5 Iverfeldt, P., Serfozo, P., Diaz Arnesto, L. and Bartfai, T., Dif-ferential release of coexisting neurotransmitters: frequency de-

Keywords

  • Calcitonin gene-related peptide
  • Carrageenan
  • Dorsal horn
  • Inflammation
  • Pain
  • Spinal cord
  • Substance P

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