In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.
Bibliographical noteFunding Information:
The authors wish to thank Dr. Michael Vasko for his generous assistance with developing the superfusion technique. This research was supported in part by NRSA Fellowship F32-DEO5606 (M.G.G.) 3 Fitzgerald, M., Capsaicin sensitive neurons. A review, Pain, 15 (1983) 145-160. 4 Hargreaves, K., Dubner, R., Brown, E, Flores, C. and Joris, J., A new and sensitive method for measuring thermal nocicep-tion in cutaneous hyperalgesia, Pain, 32 (1988) 77-78. 5 Iverfeldt, P., Serfozo, P., Diaz Arnesto, L. and Bartfai, T., Dif-ferential release of coexisting neurotransmitters: frequency de-
- Calcitonin gene-related peptide
- Dorsal horn
- Spinal cord
- Substance P