Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

H. S. Kim; J. Kwang; I. J. Yoon; H. S. Joo; M. L. Frey

doi:10.1007/BF01313785

Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

H. S. Kim, J. Kwang, I. J. Yoon, H. S. Joo, M. L. Frey

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

496 Scopus citations

Abstract

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10^8.5, 10^3.5, and 10^2.5 tissue culture infective dose 50 (TCID₅₀)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.

Original language	English (US)
Pages (from-to)	477-483
Number of pages	7
Journal	Archives of Virology
Volume	133
Issue number	3-4
DOIs	https://doi.org/10.1007/BF01313785
State	Published - Sep 1 1993

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title = "Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line",

abstract = "Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.",

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AU - Frey, M. L.

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