TY - JOUR
T1 - Enzymatic degradation of chlorodiamino-s-triazine
AU - Seffernick, Jennifer L.
AU - Shapir, Nir
AU - Schoeb, Michael
AU - Johnson, Gilbert
AU - Sadowsky, Michael J.
AU - Wackett, Lawrence P.
PY - 2002/9
Y1 - 2002/9
N2 - 2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4-amino-6-hydroxy-s-triazine (CAOT). CAOT underwent dechlorination via hydroxyatrazine ethylaminohydrolase (AtzB) to yield ammelide. This represents a newly discovered dechlorination reaction for AtzB. Ammelide was subsequently hydrolyzed by N-isopropylammelide isopropylaminohydrolase to produce cyanuric acid, a compound metabolized by a variety of soil bacteria.
AB - 2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4-amino-6-hydroxy-s-triazine (CAOT). CAOT underwent dechlorination via hydroxyatrazine ethylaminohydrolase (AtzB) to yield ammelide. This represents a newly discovered dechlorination reaction for AtzB. Ammelide was subsequently hydrolyzed by N-isopropylammelide isopropylaminohydrolase to produce cyanuric acid, a compound metabolized by a variety of soil bacteria.
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U2 - 10.1128/AEM.68.9.4672-4675.2002
DO - 10.1128/AEM.68.9.4672-4675.2002
M3 - Article
C2 - 12200330
AN - SCOPUS:0036727054
SN - 0099-2240
VL - 68
SP - 4672
EP - 4675
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 9
ER -