Abstract
Efficient conversion of carbon dioxide is of great interests to today's endeavors in controlling greenhouse gas emission. A multienzyme catalytic system that uses carbon dioxide and ethanol to produce L-lactate was demonstrated in this work, thereby providing a novel reaction route to convert bio-based ethanol to an important building block for synthesis biodegradable polymers. The synthetic route has a unique internal cofactor regeneration cycle, eliminating the need of additional chemical or energy for cofactor regeneration. Lactate was successfully synthesized with 41% of ethanol converted in a batch reaction, while a turnover number of 2.2 day-1 was reached for cofactor regeneration in a reaction with continuous feeding of ethanol. A kinetic model developed based on reaction kinetic parameters determined separately for each reaction step predicted well the reaction rates and yields of the multienzyme reaction system. Biotechnol. Bioeng. 2011;108: 465-469.
Original language | English (US) |
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Pages (from-to) | 465-469 |
Number of pages | 5 |
Journal | Biotechnology and bioengineering |
Volume | 108 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2011 |
Keywords
- Carbon dioxide
- Cofactor regeneration
- Enzymatic biocatalysis
- L-lactic acid
- Lactate dehydrogenase