(-)-epigallocatechin-3-gallate inhibition of epstein-barr virus spontaneous lytic infection involves ERK1/2 and PI3-K/Akt signaling in EBV-positive cells

Sufang Liu, Hongde Li, Lin Chen, Lifang Yang, Lili Li, Yongguan Tao, Wei Li, Zijian Li, Haidan Liu, Min Tang, Ann M. Bode, Zigang Dong, Ya Cao

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Epstein-Barr virus (EBV) reactivation into the lytic cycle plays certain roles in the development of EBV-associated diseases, including nasopharyngeal carcinoma and lymphoma. In this study, we investigated the effects of the tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) on EBV spontaneous lytic infection and the mechanism(s) involved in EBV-positive cells. We found that EGCG could effectively inhibit the constitutive lytic infection of EBV at the DNA, gene transcription and protein levels by decreasing the phosphorylation and activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt. By using cellular signaling pathway-specific inhibitors, we also explored the signaling mechanisms underlying the inhibitory effects of EGCG on EBV spontaneous lytic infection in cell models. Results show that specific inhibitors of Mitogen-Activated Protein Kinase Kinase (MEK) (PD98059) and phosphatidylinositol 3-kinase [PI3-K (LY294002)] markedly downregulated gene transcription and expression of BZLF1 and BMRF1 indicating that the MEK/ERK1/2 and PI3-K/Akt pathways are involved in the EBV spontaneous lytic cycle cascade. Therefore, one of the mechanisms by which EGCG inhibits EBV spontaneous lytic infection appears to involve the suppression of the activation of MEK/ERK1/2 and PI3-K/Akt signaling.

Original languageEnglish (US)
Pages (from-to)627-637
Number of pages11
JournalCarcinogenesis
Volume34
Issue number3
DOIs
StatePublished - Mar 2013

Bibliographical note

Funding Information:
National Nature Science Foundation of China (No. 81101474, 81072220 and 81161120410); State Key Basic Research and Development Plan (973) of the Ministry of Science and Technology of China (No. 2011CB504300); the Joint Research Fund of National Nature Science Foundation (No. R37CA081064).

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