Epithelial adhesion and expression of the integrin analog, a putative candidal adhesin, were correlated for 33 clinical and laboratory isolates of Candida albicans, other Candida species, and Saccharomyces cerevisiae. On flow cytometry with saturating concentrations of the monoclonal antibody OKM1, surface fluorescence was highest for C. albicans at 67.8% ± 1.7% and significantly reduced for Candida tropicalis (32.0% ± 2.6%), Candida parapsilosis (18.3% ± 2.4%), Candida glabrata (3.3% ± 0.8%), Candida lusitaniae (2.9% ± 1.0%), Candida krusei (0.7% ± 0.1%), and Saccharomyces cerevisiae (1.7% ± 0.2%) (P <.006 for all other species vs. C. albicans). Adhesion to a human epithelial cell line was highest for C. albicans at 49.8% ± 3.5%, lower for C. tropicalis (44.7% ± 4.6%), and incrementally reduced for all other species (<25%) (P <.012). The correlation between integrin expression and epithelial adhesion was highly significant (P =.0066; r2 =.8). Surface expression of the integrin analog predicts epithelial adhesion for yeast species isolated in opportunistic infections.
Bibliographical noteFunding Information:
Received 8 August 1994; revised 13 January 1995. Presented in part: Society for Pediatric Research meeting. New Orleans. 29 April-2 May 1991. Grant support: National Institutes of Health (AI-25827) and Pediatric AIDS Foundation (to M.K.H. and CoM.B.). Reprints or correspondence: Dr. Catherine M. Bendel. UMHC Box 296. University of Minnesota. 420 Delaware St. S.E.. Minneapolis. MN 55455.