Escherichia coli catabolite gene activator protein mutants defective in positive control of lac operon transcription

A. C. Eschenlauer, W. S. Reznikoff

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

We isolated three Escherichia coli catabolite gene activator protein mutants that are defective in the positive control of transcription initiation from the lac operon promoter region yet retain negative control of transcription from other promoters. One mutant has a substitution of valine for glutamate at residue 72, which lies in the cyclic AMP binding domain and contacts cyclie AMP. The other two mutants have substitutions of asparagine and cysteine for glycine 162, which lies in a surface-exposed turn of the DNA-binding domain. Surprisingly, although all three mutants can repress the lacP2/P3 promoters through the catabolite gene activator protein target site of lac, none displays strong dominance over the ability of wild-type catabolite gene activator protein to stimulate the lacP1 promoter.

Original languageEnglish (US)
Pages (from-to)5024-5029
Number of pages6
JournalJournal of bacteriology
Volume173
Issue number16
DOIs
StatePublished - 1991
Externally publishedYes

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