TY - JOUR
T1 - Estrogen receptor-alpha polymorphism in patients with and without degenerative disease of the temporomandibular joint
AU - Stemig, Melissa
AU - Myers, Sandra L
AU - Kaimal, Shanti
AU - Islam, Mohammad Saiful
N1 - Publisher Copyright:
© W. S. Maney & Son Ltd 2015.
PY - 2015
Y1 - 2015
N2 - Aims: The genetic aspect of degenerative joint disease (DJD) of the temporomandibular joint is poorly understood. The prevalence of the estrogen receptor alpha (ER-alpha) gene polymorphism in patients with and without DJD using xbal and pvull restriction fragment length polymorphisms (RFLPs) was studied. Methodology: DNA samples from 42 DJD+ and 36 DJD- subjects were amplified. A 346-base pair long ERalpha gene fragment containing the two sites of polymorphism in intron 1 was analyzed for xbal and pvull RFLP. Statistical analysis was carried out using Fisher’s exact test and two-group t-test. Results: Five different ER-alpha genotypes were found in both groups. These were PXPX, pxpx, pxPX, PxPX, and pxPx. Discussion: There was a higher number of pxpx and pxPX genotypes in the DJD+ samples compared to the DJD- group, which suggests the presence of polymorphism possibly modulates the ER-alpha activity in bone and contributes to the degenerative process in the joint.
AB - Aims: The genetic aspect of degenerative joint disease (DJD) of the temporomandibular joint is poorly understood. The prevalence of the estrogen receptor alpha (ER-alpha) gene polymorphism in patients with and without DJD using xbal and pvull restriction fragment length polymorphisms (RFLPs) was studied. Methodology: DNA samples from 42 DJD+ and 36 DJD- subjects were amplified. A 346-base pair long ERalpha gene fragment containing the two sites of polymorphism in intron 1 was analyzed for xbal and pvull RFLP. Statistical analysis was carried out using Fisher’s exact test and two-group t-test. Results: Five different ER-alpha genotypes were found in both groups. These were PXPX, pxpx, pxPX, PxPX, and pxPx. Discussion: There was a higher number of pxpx and pxPX genotypes in the DJD+ samples compared to the DJD- group, which suggests the presence of polymorphism possibly modulates the ER-alpha activity in bone and contributes to the degenerative process in the joint.
KW - Degenerative joint disease
KW - Estrogen receptor alpha
KW - Polymorphism
KW - Temporomandibular joint
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U2 - 10.1179/2151090314Y.0000000013
DO - 10.1179/2151090314Y.0000000013
M3 - Article
C2 - 25178548
AN - SCOPUS:84928946638
SN - 0886-9634
VL - 33
SP - 129
EP - 133
JO - Cranio - Journal of Craniomandibular Practice
JF - Cranio - Journal of Craniomandibular Practice
IS - 2
ER -