TY - JOUR
T1 - Ethanol-induced FOS immunoreactivity in the brain of μ-opioid receptor knockout mice
AU - Kolodziejska-Akiyama, Karolina M.
AU - Young, May Cha
AU - Jiang, Yuhui
AU - Loh, Horace H.
AU - Chang, Sulie L.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/11/1
Y1 - 2005/11/1
N2 - Using μ-opioid receptor knockout (MKO) mice, we examined ethanol-induced FOS immunoreactivity (FOSir) in the brain as an indicator of neuronal activation to assess the role of the μ-opioid receptor in modulating ethanol's actions in the central nervous system (CNS). Saline stimulated FOSir in the paraventricular thalamic nucleus (PVA) and the dorsal hypothalamic area (DA) in MKO mice, but not in wild-type (WT), suggesting that MKO homozygotes may differ responsively from WT. Treatment with ethanol (4 g/kg, i.p.) induced FOSir in the PVA, DA, supraoptic (SO), paraventricular hypothalamic (PVN), lateral parabrachial (LPB), locus coeruleus (LC) and Edinger-Westphal (EW) nuclei in both MKO and WT mice. However, ethanol stimulated modest FOSir in the lateral septal division (LSV), suprachiasmatic nucleus (SCh) and the dorsal and ventral lateral geniculate nuclei (DLG and VLG) in WT mice, but not in MKO mice. In contrast, higher levels of ethanol-induced FOSir were observed in the ventral pallidum (VP) and globus pallidus (GP) of MKO mice as compared to WT. These data suggest that ethanol continues to activate several brain regions, even without the μ-opioid receptor pathway. However, the μ-opioid receptor may be significant in mediating ethanol's effects in some restricted areas of the brain.
AB - Using μ-opioid receptor knockout (MKO) mice, we examined ethanol-induced FOS immunoreactivity (FOSir) in the brain as an indicator of neuronal activation to assess the role of the μ-opioid receptor in modulating ethanol's actions in the central nervous system (CNS). Saline stimulated FOSir in the paraventricular thalamic nucleus (PVA) and the dorsal hypothalamic area (DA) in MKO mice, but not in wild-type (WT), suggesting that MKO homozygotes may differ responsively from WT. Treatment with ethanol (4 g/kg, i.p.) induced FOSir in the PVA, DA, supraoptic (SO), paraventricular hypothalamic (PVN), lateral parabrachial (LPB), locus coeruleus (LC) and Edinger-Westphal (EW) nuclei in both MKO and WT mice. However, ethanol stimulated modest FOSir in the lateral septal division (LSV), suprachiasmatic nucleus (SCh) and the dorsal and ventral lateral geniculate nuclei (DLG and VLG) in WT mice, but not in MKO mice. In contrast, higher levels of ethanol-induced FOSir were observed in the ventral pallidum (VP) and globus pallidus (GP) of MKO mice as compared to WT. These data suggest that ethanol continues to activate several brain regions, even without the μ-opioid receptor pathway. However, the μ-opioid receptor may be significant in mediating ethanol's effects in some restricted areas of the brain.
KW - Ethanol
KW - Knockout mice
KW - μ-Opioid receptor
UR - http://www.scopus.com/inward/record.url?scp=26444440497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26444440497&partnerID=8YFLogxK
U2 - 10.1016/j.drugalcdep.2005.02.006
DO - 10.1016/j.drugalcdep.2005.02.006
M3 - Article
C2 - 15893889
AN - SCOPUS:26444440497
SN - 0376-8716
VL - 80
SP - 161
EP - 168
JO - Drug and alcohol dependence
JF - Drug and alcohol dependence
IS - 2
ER -