TY - GEN
T1 - Evaluation in a continuous-flow column of different fermenting substrates for the reductive dehalogenation of trichloroethene
AU - Azizian, M.
AU - Marshall, I.
AU - Behrens, S.
AU - Spormann, A.
AU - Semprini, L.
PY - 2011
Y1 - 2011
N2 - A continuous-flow column study was conducted to analyse the reductive dehalogenation of trichloroethene (TCE) in aquifer material with a composition high in iron oxides. The column was bioaugmented with the Point Mugu (PM) culture, which is a mixed microbial enrichment culture capable of completely transforming TCE to ethene (ETH). Most of the metabolic reducing equivalents were channelled to sulfate, Fe(III), and Mn(IV) reduction. When equal electron-reducing equivalents were added, the most effective dehalogenation was achieved using formate as an electron donor, with 14% of the electron equivalents going towards dehalogenation reactions, compared to 6.7% for lactate and 9.6% for propionate. Lactate and propionate addition resulted in a significant increase in Geobacter, Spirochaetes, and Desulfovibrio phylotypes relative to Dehalococcides when compared to formate addition. Molecular results support chemical observations that a greater percentage of the electron donor addition was channeled to Fe(III) reduction when lactate and propionate were added compared to formate.
AB - A continuous-flow column study was conducted to analyse the reductive dehalogenation of trichloroethene (TCE) in aquifer material with a composition high in iron oxides. The column was bioaugmented with the Point Mugu (PM) culture, which is a mixed microbial enrichment culture capable of completely transforming TCE to ethene (ETH). Most of the metabolic reducing equivalents were channelled to sulfate, Fe(III), and Mn(IV) reduction. When equal electron-reducing equivalents were added, the most effective dehalogenation was achieved using formate as an electron donor, with 14% of the electron equivalents going towards dehalogenation reactions, compared to 6.7% for lactate and 9.6% for propionate. Lactate and propionate addition resulted in a significant increase in Geobacter, Spirochaetes, and Desulfovibrio phylotypes relative to Dehalococcides when compared to formate addition. Molecular results support chemical observations that a greater percentage of the electron donor addition was channeled to Fe(III) reduction when lactate and propionate were added compared to formate.
KW - Chlorinated solvents
KW - Column study
KW - Electron donors
KW - Reductive dehalogenation
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M3 - Conference contribution
AN - SCOPUS:84860567179
SN - 9781907161162
T3 - IAHS-AISH Publication
SP - 209
EP - 212
BT - GQ10
T2 - 7th International Groundwater Quality Conference: Groundwater Quality Management in a Rapidly Changing World, GQ10
Y2 - 13 June 2010 through 18 June 2010
ER -