Evaluation of an inhibitor assay to determine serum isoamylase distribution

Carol Ellis; Donald F. Koehler; John H. Eckfeldt; Michael D. Levitt

doi:10.1007/BF01316573

Evaluation of an inhibitor assay to determine serum isoamylase distribution

Carol Ellis, Donald F. Koehler, John H. Eckfeldt, Michael D. Levitt

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We tested a technique to distinguish salivary from pancreatic isoamylase using a wheat protein which inhibits salivary isoamylase. The inhibitor technique accurately reflected the preponderance of pancreatic or salivary isoamylase in sera which had been "spiked" with human pancreatic or salivary isoamylase. Comparison of the results of either cellulose acetate electrophoresis or isoelectric focusing showed an excellent correlation (r=0.99) in 43 hyperamylasemic sera which did not contain macroamylase. Normal values (1 sd) of total serum amylase activity and the upper limit of normal for serum pancreatic isoamylase was 166 IU/liter. The inhibitor assay provides a simple and accurate means of differentiating salivary from pancreatic hyperamylasemia.

Original language	English (US)
Pages (from-to)	897-901
Number of pages	5
Journal	Digestive Diseases and Sciences
Volume	27
Issue number	10
DOIs	https://doi.org/10.1007/BF01316573
State	Published - Oct 1 1982

Access

10.1007/BF01316573

Fingerprint Dive into the research topics of 'Evaluation of an inhibitor assay to determine serum isoamylase distribution'. Together they form a unique fingerprint.

Cite this

@article{f9b9f79cb11c4af48482a2b996f8583c,

title = "Evaluation of an inhibitor assay to determine serum isoamylase distribution",

abstract = "We tested a technique to distinguish salivary from pancreatic isoamylase using a wheat protein which inhibits salivary isoamylase. The inhibitor technique accurately reflected the preponderance of pancreatic or salivary isoamylase in sera which had been {"}spiked{"} with human pancreatic or salivary isoamylase. Comparison of the results of either cellulose acetate electrophoresis or isoelectric focusing showed an excellent correlation (r=0.99) in 43 hyperamylasemic sera which did not contain macroamylase. Normal values (1 sd) of total serum amylase activity and the upper limit of normal for serum pancreatic isoamylase was 166 IU/liter. The inhibitor assay provides a simple and accurate means of differentiating salivary from pancreatic hyperamylasemia.",

author = "Carol Ellis and Koehler, {Donald F.} and Eckfeldt, {John H.} and Levitt, {Michael D.}",

year = "1982",

month = oct,

day = "1",

doi = "10.1007/BF01316573",

language = "English (US)",

volume = "27",

pages = "897--901",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer New York",

number = "10",

}

TY - JOUR

T1 - Evaluation of an inhibitor assay to determine serum isoamylase distribution

AU - Ellis, Carol

AU - Koehler, Donald F.

AU - Eckfeldt, John H.

AU - Levitt, Michael D.

PY - 1982/10/1

Y1 - 1982/10/1

N2 - We tested a technique to distinguish salivary from pancreatic isoamylase using a wheat protein which inhibits salivary isoamylase. The inhibitor technique accurately reflected the preponderance of pancreatic or salivary isoamylase in sera which had been "spiked" with human pancreatic or salivary isoamylase. Comparison of the results of either cellulose acetate electrophoresis or isoelectric focusing showed an excellent correlation (r=0.99) in 43 hyperamylasemic sera which did not contain macroamylase. Normal values (1 sd) of total serum amylase activity and the upper limit of normal for serum pancreatic isoamylase was 166 IU/liter. The inhibitor assay provides a simple and accurate means of differentiating salivary from pancreatic hyperamylasemia.

AB - We tested a technique to distinguish salivary from pancreatic isoamylase using a wheat protein which inhibits salivary isoamylase. The inhibitor technique accurately reflected the preponderance of pancreatic or salivary isoamylase in sera which had been "spiked" with human pancreatic or salivary isoamylase. Comparison of the results of either cellulose acetate electrophoresis or isoelectric focusing showed an excellent correlation (r=0.99) in 43 hyperamylasemic sera which did not contain macroamylase. Normal values (1 sd) of total serum amylase activity and the upper limit of normal for serum pancreatic isoamylase was 166 IU/liter. The inhibitor assay provides a simple and accurate means of differentiating salivary from pancreatic hyperamylasemia.

UR - http://www.scopus.com/inward/record.url?scp=0019948340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019948340&partnerID=8YFLogxK

U2 - 10.1007/BF01316573

DO - 10.1007/BF01316573

M3 - Article

C2 - 6180877

AN - SCOPUS:0019948340

VL - 27

SP - 897

EP - 901

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 10

ER -