Evaluation of biological and enzymatic quorum quencher coating additives to reduce biocorrosion of steel

Siqian Huang, Celine Bergonzi, Michael Schwab, Mikael H Elias, Randall E Hicks

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Microbial colonization can be detrimental to the integrity of metal surfaces and lead to microbiologically influenced corrosion (MIC). Biocorrosion is a serious problem for aquatic and marine industries in the world. In Minnesota (USA), where this study was conducted, biocorrosion severely affects the maritime transportation industry. The anticorrosion activity of a variety of compounds, including chemical (magnesium peroxide) and biological (surfactin, capsaicin, and gramicidin) molecules were investigated as coating additives. We also evaluated a previously engineered, extremely stable, non-biocidal enzyme known to interfere in bacterial signaling, SsoPox (a quorum quenching lactonase). Experimental steel coupons were submerged in water from the Duluth Superior Harbor (DSH) for 8 weeks in the laboratory. Biocorrosion was evaluated by counting the number and the coverage of corrosion tubercles on coupons and also by ESEM imaging of the coupon surface. Three experimental coating additives significantly reduced the formation of corrosion tubercles: surfactin, magnesium peroxide and the quorum quenching lactonase by 31%, 36% and 50%, respectively. DNA sequence analysis of the V4 region of the bacterial 16S rRNA gene revealed that these decreases in corrosion were associated with significant changes in the composition of bacterial communities on the steel surfaces. These results demonstrate the potential of highly stable quorum quenching lactonases to provide a reliable, cost-effective method to treat steel structures and prevent biocorrosion.

Original languageEnglish (US)
Article numbere0217059
JournalPloS one
Volume14
Issue number5
DOIs
StatePublished - May 2019

Bibliographical note

Funding Information:
This research was supported by funding from the Minnesota Sea Grant Program to REH and SH with additional funding from University of Minnesota MnDRIVE Initiative postdoctoral fellowship grants to REH and ME. https://mndrive. umn.edu/. The work by REH and SH used federal funds under award NA14OAR4170080 from Minnesota Sea Grant, National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce. The statements, findings, conclusions, and recommendations are those of the author(s) and do not necessarily reflect the views of NOAA, the Sea Grant College Program, or the U.S. Department of Commerce. This paper is journal reprint No. JR658 of the Minnesota Sea Grant College Program. http://www.seagrant.umn.edu/. Disclaimer: The statements, findings, conclusions, and recommendations are those of the author(s) and do not necessarily reflect the views of NOAA, the Sea Grant College Program, or the U.S. Department of Commerce. The authors wish to thank AMI Consulting Engineers for providing steel coupons, the Natural Resources Research Institute for use of the Hitachi ESEM, and Anqi Zhao for assistance in graphs coding with R. This research was supported by funding from the Minnesota Sea Grant Program to REH and SH with additional funding from a University of Minnesota MnDRIVE Initiative postdoctoral fellowship grant to REH and ME. The work by REH and SH used federal funds under award NA14OAR4170080 from Minnesota Sea Grant, National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce. This paper is journal reprint No. JR658 of the Minnesota Sea Grant College Program.

Publisher Copyright:
© 2019 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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