Evaluation of clinicopathological characteristics and oestrogen receptor gene expression in oestrogen receptor-negative, progesterone receptor-positive canine mammary carcinomas

N. H. Kim; H. Y. Lim; K. S. Im; J. I. Shin; H. W. Kim; J. H. Sur

doi:10.1016/j.jcpa.2014.04.001

Evaluation of clinicopathological characteristics and oestrogen receptor gene expression in oestrogen receptor-negative, progesterone receptor-positive canine mammary carcinomas

N. H. Kim, H. Y. Lim, K. S. Im, J. I. Shin, H. W. Kim, J. H. Sur

Veterinary Clinical Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The existence of the oestrogen receptor-negative (OR^-)/progesterone receptor-positive (PR⁺) phenotype in canine mammary carcinomas (CMCs) is not well understood, although this phenotype was reported consistently in previous studies. In the present study, immunohistochemistry (IHC) was performed to categorize CMCs with the OR^-/PR⁺ phenotype and compare their clinicopathological features with OR⁺/PR⁺ tumours. Of a total of 305 CMCs, 36 (11.8%) were categorized as OR^-/PR⁺ and showed intermediate characteristics between those of OR⁺/PR⁺ and OR^-/PR^- cases. OR mRNA levels were measured in formalin-fixed, paraffin wax-embedded samples using a novel branched-chain DNA assay method. Similar to the IHC result, one-way analysis of variance showed that the mean normalized OR mRNA level of OR^-/PR⁺ tumours (11.4±16.34) was between that of the OR^-/PR^- (mean 4.7±6.35) and OR⁺/PR⁺ (mean 15.8±11.95) (P=0.033) tumours. Only three of the 36 OR^-/PR⁺ tumours completely lacked OR mRNA expression. The OR^-/PR⁺ tumours were not categorized as an independent group nor were theyincluded in the other groups on post-hoc analysis. OR^-/PR⁺ tumours were associated with factors related to poor prognosis compared with OR⁺/PR⁺ tumours, but OR^-/PR^- tumours were associated with the worst prognostic indicators. Further studies are required in order to determine the clinical significance of the OR^-/PR⁺ phenotype.

Original language	English (US)
Pages (from-to)	42-50
Number of pages	9
Journal	Journal of Comparative Pathology
Volume	151
Issue number	1
DOIs	https://doi.org/10.1016/j.jcpa.2014.04.001
State	Published - Jul 2014

Bibliographical note

Funding Information:
The authors thank Ms. R. H. Jang for excellent technical assistance. This report summarizes work contained within a thesis submitted by N.-H. Kim to fulfil the requirements for a PhD. This research was supported by the Basic Science Research Programme of the National Research Foundation of Korea (NRF) and was funded by the Ministry of Education, Science and Technology ( 2011-0021337 ).

Keywords

Branched-chain DNA assay
Canine mammary carcinoma
Steroid receptors

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title = "Evaluation of clinicopathological characteristics and oestrogen receptor gene expression in oestrogen receptor-negative, progesterone receptor-positive canine mammary carcinomas",

abstract = "The existence of the oestrogen receptor-negative (OR-)/progesterone receptor-positive (PR+) phenotype in canine mammary carcinomas (CMCs) is not well understood, although this phenotype was reported consistently in previous studies. In the present study, immunohistochemistry (IHC) was performed to categorize CMCs with the OR-/PR+ phenotype and compare their clinicopathological features with OR+/PR+ tumours. Of a total of 305 CMCs, 36 (11.8%) were categorized as OR-/PR+ and showed intermediate characteristics between those of OR+/PR+ and OR-/PR- cases. OR mRNA levels were measured in formalin-fixed, paraffin wax-embedded samples using a novel branched-chain DNA assay method. Similar to the IHC result, one-way analysis of variance showed that the mean normalized OR mRNA level of OR-/PR+ tumours (11.4±16.34) was between that of the OR-/PR- (mean 4.7±6.35) and OR+/PR+ (mean 15.8±11.95) (P=0.033) tumours. Only three of the 36 OR-/PR+ tumours completely lacked OR mRNA expression. The OR-/PR+ tumours were not categorized as an independent group nor were theyincluded in the other groups on post-hoc analysis. OR-/PR+ tumours were associated with factors related to poor prognosis compared with OR+/PR+ tumours, but OR-/PR- tumours were associated with the worst prognostic indicators. Further studies are required in order to determine the clinical significance of the OR-/PR+ phenotype.",

keywords = "Branched-chain DNA assay, Canine mammary carcinoma, Steroid receptors",

author = "Kim, {N. H.} and Lim, {H. Y.} and Im, {K. S.} and Shin, {J. I.} and Kim, {H. W.} and Sur, {J. H.}",

note = "Funding Information: The authors thank Ms. R. H. Jang for excellent technical assistance. This report summarizes work contained within a thesis submitted by N.-H. Kim to fulfil the requirements for a PhD. This research was supported by the Basic Science Research Programme of the National Research Foundation of Korea (NRF) and was funded by the Ministry of Education, Science and Technology ( 2011-0021337 ).",

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AU - Shin, J. I.

AU - Kim, H. W.

AU - Sur, J. H.

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N2 - The existence of the oestrogen receptor-negative (OR-)/progesterone receptor-positive (PR+) phenotype in canine mammary carcinomas (CMCs) is not well understood, although this phenotype was reported consistently in previous studies. In the present study, immunohistochemistry (IHC) was performed to categorize CMCs with the OR-/PR+ phenotype and compare their clinicopathological features with OR+/PR+ tumours. Of a total of 305 CMCs, 36 (11.8%) were categorized as OR-/PR+ and showed intermediate characteristics between those of OR+/PR+ and OR-/PR- cases. OR mRNA levels were measured in formalin-fixed, paraffin wax-embedded samples using a novel branched-chain DNA assay method. Similar to the IHC result, one-way analysis of variance showed that the mean normalized OR mRNA level of OR-/PR+ tumours (11.4±16.34) was between that of the OR-/PR- (mean 4.7±6.35) and OR+/PR+ (mean 15.8±11.95) (P=0.033) tumours. Only three of the 36 OR-/PR+ tumours completely lacked OR mRNA expression. The OR-/PR+ tumours were not categorized as an independent group nor were theyincluded in the other groups on post-hoc analysis. OR-/PR+ tumours were associated with factors related to poor prognosis compared with OR+/PR+ tumours, but OR-/PR- tumours were associated with the worst prognostic indicators. Further studies are required in order to determine the clinical significance of the OR-/PR+ phenotype.

AB - The existence of the oestrogen receptor-negative (OR-)/progesterone receptor-positive (PR+) phenotype in canine mammary carcinomas (CMCs) is not well understood, although this phenotype was reported consistently in previous studies. In the present study, immunohistochemistry (IHC) was performed to categorize CMCs with the OR-/PR+ phenotype and compare their clinicopathological features with OR+/PR+ tumours. Of a total of 305 CMCs, 36 (11.8%) were categorized as OR-/PR+ and showed intermediate characteristics between those of OR+/PR+ and OR-/PR- cases. OR mRNA levels were measured in formalin-fixed, paraffin wax-embedded samples using a novel branched-chain DNA assay method. Similar to the IHC result, one-way analysis of variance showed that the mean normalized OR mRNA level of OR-/PR+ tumours (11.4±16.34) was between that of the OR-/PR- (mean 4.7±6.35) and OR+/PR+ (mean 15.8±11.95) (P=0.033) tumours. Only three of the 36 OR-/PR+ tumours completely lacked OR mRNA expression. The OR-/PR+ tumours were not categorized as an independent group nor were theyincluded in the other groups on post-hoc analysis. OR-/PR+ tumours were associated with factors related to poor prognosis compared with OR+/PR+ tumours, but OR-/PR- tumours were associated with the worst prognostic indicators. Further studies are required in order to determine the clinical significance of the OR-/PR+ phenotype.

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KW - Steroid receptors

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Evaluation of clinicopathological characteristics and oestrogen receptor gene expression in oestrogen receptor-negative, progesterone receptor-positive canine mammary carcinomas

Abstract

Bibliographical note

Keywords

UN SDGs

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