Evaluation of colorimetric assays for determination of H                         2                         O                         2                          in planta during fungal wood decomposition

Jesus D. Castaño; Jiwei Zhang; Jonathan S. Schilling

doi:10.1016/j.mimet.2017.12.004

Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition

Jesus D. Castaño, Jiwei Zhang, Jonathan S. Schilling

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Hydrogen peroxide (H ₂ O ₂ ) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.

Original language	English (US)
Pages (from-to)	10-13
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	145
DOIs	https://doi.org/10.1016/j.mimet.2017.12.004
State	Published - Feb 2018

Bibliographical note

Funding Information:
We thank the Fulbright commission, Colciencias, and the University of Minnesota for the funding of the researchers.

Keywords

Amplex red 10
Brown rot
Fungi
Radical

Access

10.1016/j.mimet.2017.12.004

OpenUrl availability

Full text

Cite this

Castaño, J. D., Zhang, J., & Schilling, J. S. (2018). Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition Journal of Microbiological Methods, 145, 10-13. https://doi.org/10.1016/j.mimet.2017.12.004

Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition . / Castaño, Jesus D.; Zhang, Jiwei ; Schilling, Jonathan S.
In: Journal of Microbiological Methods, Vol. 145, 02.2018, p. 10-13.

Research output: Contribution to journal › Article › peer-review

Castaño, JD, Zhang, J & Schilling, JS 2018, ' Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition ', Journal of Microbiological Methods, vol. 145, pp. 10-13. https://doi.org/10.1016/j.mimet.2017.12.004

Castaño JD, Zhang J , Schilling JS. Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition Journal of Microbiological Methods. 2018 Feb;145:10-13. doi: 10.1016/j.mimet.2017.12.004

Castaño, Jesus D. ; Zhang, Jiwei ; Schilling, Jonathan S. / Evaluation of colorimetric assays for determination of H ₂ O ₂ in planta during fungal wood decomposition In: Journal of Microbiological Methods. 2018 ; Vol. 145. pp. 10-13.

@article{d3d12400834145938dbe4de9f8224a39,

title = " Evaluation of colorimetric assays for determination of H 2 O 2 in planta during fungal wood decomposition ",

abstract = " Hydrogen peroxide (H 2 O 2 ) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity. ",

keywords = "Amplex red 10, Brown rot, Fungi, Radical",

author = "Casta{\~n}o, {Jesus D.} and Jiwei Zhang and Schilling, {Jonathan S.}",

note = "Funding Information: We thank the Fulbright commission, Colciencias, and the University of Minnesota for the funding of the researchers.",

year = "2018",

month = feb,

doi = "10.1016/j.mimet.2017.12.004",

language = "English (US)",

volume = "145",

pages = "10--13",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

TY - JOUR

T1 - Evaluation of colorimetric assays for determination of H 2 O 2 in planta during fungal wood decomposition

AU - Castaño, Jesus D.

AU - Zhang, Jiwei

AU - Schilling, Jonathan S.

N1 - Funding Information: We thank the Fulbright commission, Colciencias, and the University of Minnesota for the funding of the researchers.

PY - 2018/2

Y1 - 2018/2

N2 - Hydrogen peroxide (H 2 O 2 ) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.

AB - Hydrogen peroxide (H 2 O 2 ) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.

KW - Amplex red 10

KW - Brown rot

KW - Fungi

KW - Radical

UR - http://www.scopus.com/inward/record.url?scp=85037855716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037855716&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2017.12.004

DO - 10.1016/j.mimet.2017.12.004

M3 - Article

C2 - 29242076

AN - SCOPUS:85037855716

SN - 0167-7012

VL - 145

SP - 10

EP - 13

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -