Evaluation of Escherichia coli isolates from healthy chickens to determine their potential risk to poultry and human health

Zachary R. Stromberg, James R. Johnson, John M. Fairbrother, Jacquelyn Kilbourne, Angelica Van Goor, Roy Curtiss, Melha Mellata

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43 Scopus citations

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) strains are important pathogens that cause diverse diseases in humans and poultry. Some E. coli isolates from chicken feces contain ExPEC-associated virulence genes, so appear potentially pathogenic; they conceivably could be transmitted to humans through handling and/or consumption of contaminated meat. However, the actual extraintestinal virulence potential of chicken-source fecal E. coli is poorly understood. Here, we assessed whether fecal E. coli isolates from healthy production chickens could cause diseases in a chicken model of avian colibacillosis and three rodent models of ExPEC-associated human infections. From 304 E. coli isolates from chicken fecal samples, 175 E. coli isolates were screened by PCR for virulence genes associated with human-source ExPEC or avian pathogenic E. coli (APEC), an ExPEC subset that causes extraintestinal infections in poultry. Selected isolates genetically identified as ExPEC and non-ExPEC isolates were assessed in vitro for virulence-associated phenotypes, and in vivo for disease-causing ability in animal models of colibacillosis, sepsis, meningitis, and urinary tract infection. Among the study isolates, 13% (40/304) were identified as ExPEC; the majority of these were classified as APEC and uropathogenic E. coli, but none as neonatal meningitis E. coli. Multiple chicken-source fecal ExPEC isolates resembled avian and human clinical ExPEC isolates in causing one or more ExPEC-associated illnesses in experimental animal infection models. Additionally, some isolates that were classified as non-ExPEC were able to cause ExPEC-associated illnesses in animal models, and thus future studies are needed to elucidate their mechanisms of virulence. These findings show that E. coli isolates from chicken feces contain ExPEC-associated genes, exhibit ExPEC-associated in vitro phenotypes, and can cause ExPEC-associated infections in animal models, and thus may pose a health threat to poultry and consumers.

Original languageEnglish (US)
Article numbere0180599
JournalPloS one
Volume12
Issue number7
DOIs
StatePublished - Jul 2017

Bibliographical note

Funding Information:
This research was supported by a grant from the U.S. Department of Agriculture National Research Initiative USDA-NIFA-AFRI grant 2011-67005-30182 (M.M. and R.C.), and by Office of Research and Development, Medical Research Service, Department of Veterans Affairs grant #1 I01 CX000192 01 (J.R.J.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors thank Mr. Brian Johnston (Veterans Affairs Medical Center and University of Minnesota), Ms. Natalie Mitchell (Biodesign Institute, Arizona State University), and Mr. Jocelyn Bernier-Lachance and Ms. Ghyslaine Vanier (Faculty of Veterinary Medicine, Universite ? de Montre?al) for technical assistance.

Publisher Copyright:
© 2017 Stromberg et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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