Evaluation of neurobehavioral abnormalities and immunotoxicity in response to oral imidacloprid exposure in domestic chickens (Gallus gallus domesticus)

Dana Franzen-Klein, Mark Jankowski, Charlotte L. Roy, Hoa Nguyen-Phuc, Da Chen, Lorin Neuman-Lee, Patrick Redig, Julia Ponder

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Domestic chickens (Gallus gallus domesticus) were exposed to imidacloprid by gavage once daily for 7 consecutive days at 0, 0.03, 0.34, 3.42, 10.25, and 15.5 mg/kg/day (n = 20 per group; 5 6-week-old males, 5 6-week-old females, 5 9-week-old males, and 5 9-week-old females). The severity and duration of neurobehavioral abnormalities were recorded. Components of the innate and adaptive immune system were assessed with 7 standard functional assays. Temporary neurobehavioral abnormalities were observed in a dose-dependent manner, including muscle tremors, ataxia, and depressed mentation. Based upon mean clinical severity scores, the no observed adverse effect level (NOAEL) was 3.42 mg/kg/day, and the lowest observed adverse effect level (LOAEL) was 10.25 mg/kg/day. The effective dose value for the presence of any neurobehavioral abnormalities in 50% of the test group (ED50) was 4.62 ± 0.98 mg/kg/day. The ED50 for an adjusted score that included both severity and duration of neurobehavioral abnormalities was 11.24 ± 9.33 mg/kg/day. These ED50 values are equivalent to a 1 kg bird ingesting 29 or 70 imidacloprid treated soybean seeds respectively. Immunotoxicity was not documented, possible causes include the assays were insensitive, relevant immune functions were not examined, or imidacloprid is not immunotoxic at this dosing schedule in this species. Neurobehavioral abnormalities were a more sensitive indicator of the sublethal effects of imidacloprid than immunotoxicity.

Original languageEnglish (US)
Pages (from-to)45-65
Number of pages21
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume83
Issue number2
DOIs
StatePublished - Jan 17 2020

Bibliographical note

Funding Information:
Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR). Special thanks to Carlie LaLone (United States Environmental Protection Agency) for assistance with the SeqAPASS data.

Keywords

  • Avian toxicity
  • behavioral toxicology
  • neonicotinoids
  • neurotoxicity
  • risk assessment

PubMed: MeSH publication types

  • Journal Article
  • Randomized Controlled Trial, Veterinary
  • Research Support, Non-U.S. Gov't

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