Evaluation of the iron regulatory protein-1 interactome

Gregory J Connell, Jando S. Danial, Christian X. Haastruthers

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The interactions of iron regulatory proteins (IRPs) with mRNAs containing an iron-responsive element (IRE) is a major means through which intracellular iron homeostasis is maintained and integrated with cellular function. Although IRE–IRP interactions have been proposed to modulate the expression of a diverse number of mRNAs, a transcriptome analysis of the interactions that form within the native mRNA structure and cellular environment has not previously been described. An RNA-CLIP study is described here that identified IRP-1 interactions occurring within a primary cell line expressing physiologically relevant amounts of mRNA and protein. The study suggests that only a small subset of the previously proposed IREs interact with IRP-1 in situ. Identifying authentic IRP interactions is not only important to a greater understanding of iron homeostasis and its integration with cell biology but also to the development of novel therapeutics that can compensate for iron imbalances.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalBioMetals
Volume31
Issue number1
DOIs
StatePublished - Feb 1 2018

Bibliographical note

Funding Information:
This work was funded in part by a grant from the University of Minnesota Academic Health Center to GJC (Grant Number 12.04). We also acknowledge the assistance of the University of Minnesota Genomics Center and the University of Minnesota Supercomputing Institute.

Keywords

  • Interactome
  • Iron homeostasis
  • Iron regulatory protein
  • Iron-responsive elements
  • RNA-CLIP

Fingerprint Dive into the research topics of 'Evaluation of the iron regulatory protein-1 interactome'. Together they form a unique fingerprint.

Cite this