Evidence for a μ-opioid-opioid connection between the paraventricular nucleus and ventral tegmental area in the rat

Joseph G. Quinn; Eugene O'Hare; Allen S. Levine; Eun Mee Kim

doi:10.1016/j.brainres.2003.08.020

Evidence for a μ-opioid-opioid connection between the paraventricular nucleus and ventral tegmental area in the rat

Joseph G. Quinn, Eugene O'Hare, Allen S. Levine, Eun Mee Kim

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The paraventricular nucleus (PVN) and the ventral tegmental area (VTA) have been shown to be involved in opioid mediated feeding behavior. The present study examined whether μ-opioid signalling between the PVN and VTA affected feeding behavior. Male Sprague-Dawley rats were cannulated with one cannula placed in the PVN and two cannulae placed in the VTA, which allowed for co-administration of the μ-opioid receptor agonist [D-Ala², NMe-Phe⁴, Gly-ol⁵]-enkephalin (DAMGO) in one site and the opioid antagonist naltrexone (NTX) in the other site. Bilateral administration of DAMGO (1.2, 2.4 and 4.9 nmol) into the VTA stimulated feeding dose dependently at 2.4 and 4.9 nmol (P<0.05). The DAMGO (2.4 nmol)-induced increase of food intake following injection into the PVN was blocked by bilateral injection of NTX (6.6, 13.2 and 26.5 nmol) into the VTA at 2 and 4 h (P<0.05). In the reverse situation, the DAMGO (2.4 nmol)-induced increase of food intake following injection into the VTA was blocked by injection of NTX (13.2 and 26.5 nmol) into the PVN at 2 and 4 h (P<0.05). The present study suggests that a bidirectional μ-opioid-opioid signalling pathway exists between the PVN and the VTA which influences feeding.

Original language	English (US)
Pages (from-to)	206-211
Number of pages	6
Journal	Brain Research
Volume	991
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2003.08.020
State	Published - Nov 21 2003
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Sinead Eccles, Katrina Sharkey and Sarah Strange for their assistance. This research was supported by the Wellcome Trust 063938/GM/KM/RL.

Keywords

DAMGO
Opioid
Paraventricular nucleus

Access

10.1016/j.brainres.2003.08.020

OpenUrl availability

Full text

Cite this

@article{408ba0a3758e40dca2f4b5523a12c07b,

title = "Evidence for a μ-opioid-opioid connection between the paraventricular nucleus and ventral tegmental area in the rat",

abstract = "The paraventricular nucleus (PVN) and the ventral tegmental area (VTA) have been shown to be involved in opioid mediated feeding behavior. The present study examined whether μ-opioid signalling between the PVN and VTA affected feeding behavior. Male Sprague-Dawley rats were cannulated with one cannula placed in the PVN and two cannulae placed in the VTA, which allowed for co-administration of the μ-opioid receptor agonist [D-Ala2, NMe-Phe4, Gly-ol5]-enkephalin (DAMGO) in one site and the opioid antagonist naltrexone (NTX) in the other site. Bilateral administration of DAMGO (1.2, 2.4 and 4.9 nmol) into the VTA stimulated feeding dose dependently at 2.4 and 4.9 nmol (P<0.05). The DAMGO (2.4 nmol)-induced increase of food intake following injection into the PVN was blocked by bilateral injection of NTX (6.6, 13.2 and 26.5 nmol) into the VTA at 2 and 4 h (P<0.05). In the reverse situation, the DAMGO (2.4 nmol)-induced increase of food intake following injection into the VTA was blocked by injection of NTX (13.2 and 26.5 nmol) into the PVN at 2 and 4 h (P<0.05). The present study suggests that a bidirectional μ-opioid-opioid signalling pathway exists between the PVN and the VTA which influences feeding.",

keywords = "DAMGO, Opioid, Paraventricular nucleus",

author = "Quinn, {Joseph G.} and Eugene O'Hare and Levine, {Allen S.} and Kim, {Eun Mee}",

note = "Funding Information: The authors would like to thank Sinead Eccles, Katrina Sharkey and Sarah Strange for their assistance. This research was supported by the Wellcome Trust 063938/GM/KM/RL.",

year = "2003",

month = nov,

day = "21",

doi = "10.1016/j.brainres.2003.08.020",

language = "English (US)",

volume = "991",

pages = "206--211",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Evidence for a μ-opioid-opioid connection between the paraventricular nucleus and ventral tegmental area in the rat

AU - Quinn, Joseph G.

AU - O'Hare, Eugene

AU - Levine, Allen S.

AU - Kim, Eun Mee

N1 - Funding Information: The authors would like to thank Sinead Eccles, Katrina Sharkey and Sarah Strange for their assistance. This research was supported by the Wellcome Trust 063938/GM/KM/RL.

PY - 2003/11/21

Y1 - 2003/11/21

N2 - The paraventricular nucleus (PVN) and the ventral tegmental area (VTA) have been shown to be involved in opioid mediated feeding behavior. The present study examined whether μ-opioid signalling between the PVN and VTA affected feeding behavior. Male Sprague-Dawley rats were cannulated with one cannula placed in the PVN and two cannulae placed in the VTA, which allowed for co-administration of the μ-opioid receptor agonist [D-Ala2, NMe-Phe4, Gly-ol5]-enkephalin (DAMGO) in one site and the opioid antagonist naltrexone (NTX) in the other site. Bilateral administration of DAMGO (1.2, 2.4 and 4.9 nmol) into the VTA stimulated feeding dose dependently at 2.4 and 4.9 nmol (P<0.05). The DAMGO (2.4 nmol)-induced increase of food intake following injection into the PVN was blocked by bilateral injection of NTX (6.6, 13.2 and 26.5 nmol) into the VTA at 2 and 4 h (P<0.05). In the reverse situation, the DAMGO (2.4 nmol)-induced increase of food intake following injection into the VTA was blocked by injection of NTX (13.2 and 26.5 nmol) into the PVN at 2 and 4 h (P<0.05). The present study suggests that a bidirectional μ-opioid-opioid signalling pathway exists between the PVN and the VTA which influences feeding.

AB - The paraventricular nucleus (PVN) and the ventral tegmental area (VTA) have been shown to be involved in opioid mediated feeding behavior. The present study examined whether μ-opioid signalling between the PVN and VTA affected feeding behavior. Male Sprague-Dawley rats were cannulated with one cannula placed in the PVN and two cannulae placed in the VTA, which allowed for co-administration of the μ-opioid receptor agonist [D-Ala2, NMe-Phe4, Gly-ol5]-enkephalin (DAMGO) in one site and the opioid antagonist naltrexone (NTX) in the other site. Bilateral administration of DAMGO (1.2, 2.4 and 4.9 nmol) into the VTA stimulated feeding dose dependently at 2.4 and 4.9 nmol (P<0.05). The DAMGO (2.4 nmol)-induced increase of food intake following injection into the PVN was blocked by bilateral injection of NTX (6.6, 13.2 and 26.5 nmol) into the VTA at 2 and 4 h (P<0.05). In the reverse situation, the DAMGO (2.4 nmol)-induced increase of food intake following injection into the VTA was blocked by injection of NTX (13.2 and 26.5 nmol) into the PVN at 2 and 4 h (P<0.05). The present study suggests that a bidirectional μ-opioid-opioid signalling pathway exists between the PVN and the VTA which influences feeding.

KW - DAMGO

KW - Opioid

KW - Paraventricular nucleus

UR - http://www.scopus.com/inward/record.url?scp=0142187113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142187113&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2003.08.020

DO - 10.1016/j.brainres.2003.08.020

M3 - Article

C2 - 14575893

AN - SCOPUS:0142187113

SN - 0006-8993

VL - 991

SP - 206

EP - 211

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Evidence for a μ-opioid-opioid connection between the paraventricular nucleus and ventral tegmental area in the rat

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this