Evidence for the covalent linkage of carbohydrate polymers to a glycoprotein from Streptococcus sanguis

P. R. Erickson, M. C. Herzberg

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

The platelet aggregation-associated protein (PAAP) from Streptococcus sanguis contains 39% carbohydrate in rhamnose-rich polysaccharides. Synthesized by cultured protoplasts, these polysaccharides were shown to be covalently linked to this cell wall protein using specific inhibitors of glycosylation, glycosidase treatment, amino acid and carbohydrate analyses of prepared minimal glycopeptides and isolated protein, and NMR spectroscopy. To our knowledge, this is the first direct proof of an N-asparaginyl linkage of carbohydrate to a eubacterial protein.

Original languageEnglish (US)
Pages (from-to)23780-23783
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number32
StatePublished - 1993

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