Abstract
An adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+-calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of Ca2+ calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+ calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggested that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.
Original language | English (US) |
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Pages (from-to) | 5582-5586 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 76 |
Issue number | 11 |
DOIs | |
State | Published - 1979 |