Evidence that 4-pregnen-17,20β,21-triol-3-one functions as a maturation-inducing hormone and pheromonal precursor in the percid fish, Gymnocephalus cernuus

P. W. Sorensen, C. A. Murphy, K. Loomis, P. Maniak, P. Thomas

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Behavioral, biochemical, and electrophysiological studies suggest that the trihydroxylated progestin steroid, 4-pregnen-17,20β,21-triol-3-one (20β-S) stimulates oocyte maturation and pheromone release in the Eurasian ruffe, a freshwater percid fish. Behavioral observations found that female ruffe undergoing oocyte maturation (OM) release a pheromonal cue that stimulates swimming activity and social interactions among conspecific males. Neither vitellogenic nor ovulated females released the cue. Pheromone production was directly associated with elevated plasma levels of 20β-S in maturing female ruffe which in vitro incubation suggested to be a possible maturation-inducing hormone (MIH) in this species along with 4-pregnen-17,20β-diol-3-one (17,20βP). However, neither of these steroids appear to be the pheromone because electrophysiological and behavioral studies found them to lack olfactory (EOG) and behavioral activity. Instead, studies of the odor of steroid-injected fish suggest the pheromone is a metabolite of 20β-S. In particular, inter-peritoneal injection of 20β-S (but not 17,20βP) consistently induced release of a urinary cue with strong behavioral activity. The pheromone may be a highly polar and novel metabolite because it could not be extracted using octadecylsilane resin (C18) which has proven effective for other teleost hormonal pheromones.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalGeneral and Comparative Endocrinology
Volume139
Issue number1
DOIs
StatePublished - Oct 2004

Bibliographical note

Funding Information:
Many thanks to the crew of the R.V. Coaster and the U.S. Geological Survey, Biological Research Division, Ashland, Wisconsin who collected the ruffe. We thank two anonymous reviewers for their excellent and insightful comments. P.W.S. was supported by the Minnesota Agriculture Experiment Station. This study is the result of research sponsored by the Minnesota Sea Grant College Program supported by the NOAA Office of Sea Grant, United States Department of Commerce, under grant no. USDOC-N46RG0101. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. JR496 of the Minnesota Sea Grant College Program.

Keywords

  • Behavior
  • Maturation-inducing hormone
  • Olfaction
  • Oocyte maturation
  • Pheromone

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