Abstract
The relatively new technique of high-performance liquid affinity chromatography (HPLAC) has been plagued by very large plate heights. Here we show that the large plate heights observed in the concanavalin A (Con A) HPLAC system arise from the sluggish chemical kinetics of the Con A-sugar interaction, and that other mass transfer terms contribute much less to the observed plate height. The rate parameters for the association and dissociation of various sugar derivatives from silica-bound Con A are chromatography determined. The effects of isotherm non-linearity on both the thermodynamic and kinetic properties of immobilized Con A are described. The enhancement effect of mobile phase competing sugar on the kinetics of the immobilized Con A-sugar interaction is investigated in detail, and possible reasons for this effect are proposed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-32 |
| Number of pages | 22 |
| Journal | Journal of Chromatography A |
| Volume | 357 |
| Issue number | C |
| DOIs | |
| State | Published - 1986 |
Bibliographical note
Funding Information:This work was supported by the Intramural Research Program (ES103316, ES103318, and ES103319) at the National Institute of Environmental Health Sciences, National Institutes of Health and performed for the National Toxicology Program, Public Health Service, U.S. Department of Health and Human Services under contracts HHSN273201800006C, HHSN273201600020C, HHSN273201600011C, GS00Q14OADU417 (Order No. HHSN273201600015U), HHSN273201500006C, HHSN273201500012C, HHSN273201500014C, HHSN273201400027C, HHSN273201300009C, HHSN316201200054W, HHSN273201000016C, HHS273200900002C, HHSN273200900005C, N01-ES-55551, N01-ES-55541, and N01-ES-55536.