Examination of the thermodynamic and kinetic characteristics of microparticulate affinity chromatography supports. Application to concanavalin A

Amy J. Muller, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The relatively new technique of high-performance liquid affinity chromatography (HPLAC) has been plagued by very large plate heights. Here we show that the large plate heights observed in the concanavalin A (Con A) HPLAC system arise from the sluggish chemical kinetics of the Con A-sugar interaction, and that other mass transfer terms contribute much less to the observed plate height. The rate parameters for the association and dissociation of various sugar derivatives from silica-bound Con A are chromatography determined. The effects of isotherm non-linearity on both the thermodynamic and kinetic properties of immobilized Con A are described. The enhancement effect of mobile phase competing sugar on the kinetics of the immobilized Con A-sugar interaction is investigated in detail, and possible reasons for this effect are proposed.

Original languageEnglish (US)
Pages (from-to)11-32
Number of pages22
JournalJournal of Chromatography A
Volume357
Issue numberC
DOIs
StatePublished - 1986

Fingerprint Dive into the research topics of 'Examination of the thermodynamic and kinetic characteristics of microparticulate affinity chromatography supports. Application to concanavalin A'. Together they form a unique fingerprint.

Cite this