Abstract
In this work, glucose addition (0.7 g l−1) almost doubled hydrogen yield of Chlorella pyrenoidosa (121.1 ml l−1 vs 65.5 ml l−1), with a positive correlation between hydrogen production and glucose consumption (−0.977, P < 0.01). When the electrons transport from water photolysis to algal hydrogenase was inhibited, the hydrogen productivity declined by 21.1%; whereas it dramatically decreased by 70.9% when the algal nicotinamide adeninedinucleotide dehydrogenase (NADH) was inhibited. Therefore, in the presence of glucose, the electrons for algae based hydrogen production would be mainly from glucose glycolysis rather than water photolysis. Further deuterated-glucose trial indicated that the glucose might serve as an electron donor for algal hydrogenases. Finally, a tentative electron transport route from glucose to algal hydrogenase was proposed, hoping to provide more scientific direction for further algae-based hydrogen production improvement.
Original language | English (US) |
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Article number | 121762 |
Journal | Bioresource Technology |
Volume | 289 |
DOIs | |
State | Published - Oct 2019 |
Bibliographical note
Funding Information:This research was financially supported by Zhejiang Provincial Natural Science Foundation of China ( LQ17C030001 ), Scientific Research Fund of Zhejiang Provincial Education Department ( Y201840278 ) and China Scholarship Council ( CSC ). We gratefully acknowledge all the reviewers for their great contribution to the improvement of this article.
Funding Information:
This research was financially supported by Zhejiang Provincial Natural Science Foundation of China (LQ17C030001), Scientific Research Fund of Zhejiang Provincial Education Department (Y201840278) and China Scholarship Council (CSC). We gratefully acknowledge all the reviewers for their great contribution to the improvement of this article.
Publisher Copyright:
© 2019 Elsevier Ltd
Keywords
- Algal H production
- Deuterium
- Glucose
- NADH
- Sulfur-deprivation