TY - JOUR
T1 - Experimental calcium silicate-based cement with and without zirconium oxide modulates fibroblasts viability
AU - Slompo, Camila
AU - Peres-Buzalaf, Camila
AU - Gasque, Kellen Cristina da Silva
AU - Damante, Carla Andreotti
AU - Ordinola-Zapata, Ronald
AU - Duarte, Marco Antonio Hungaro
AU - de Oliveira, Rodrigo Cardoso
N1 - Publisher Copyright:
© 2015, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
PY - 2015/11
Y1 - 2015/11
N2 - The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco’s modified Eagle’s medium (DMEM) for periods of 12, 24, and 48 h. Groups tested were white Portland cement (WPC), white Portland cement with zirconium oxide (WPCZO), and white mineral trioxide aggregate Angelus (MTA). Control group cells were not treated. The cytotoxicity was evaluated through mitochondrial-activity (MTT) and cell-density (crystal violet) assays. All cements showed low cytotoxicity. In general, at the concentration of 10 mg/mL there was an increase in viability of those groups treated with WPC and WPCZO when compared to the control group (p<0.05). A similar profile for the absorbance values was noted among the groups: 10 mg/mL presented an increase in viability compared to the control group. On the other hand, smaller concentrations presented a similar or lower viability compared to the control group, in general. A new dental material composed of calcium silicate-based cement with 20% zirconium oxide as the radiopacifier showed low cytotoxicity as a promising material to be exploited for root-end filling.
AB - The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco’s modified Eagle’s medium (DMEM) for periods of 12, 24, and 48 h. Groups tested were white Portland cement (WPC), white Portland cement with zirconium oxide (WPCZO), and white mineral trioxide aggregate Angelus (MTA). Control group cells were not treated. The cytotoxicity was evaluated through mitochondrial-activity (MTT) and cell-density (crystal violet) assays. All cements showed low cytotoxicity. In general, at the concentration of 10 mg/mL there was an increase in viability of those groups treated with WPC and WPCZO when compared to the control group (p<0.05). A similar profile for the absorbance values was noted among the groups: 10 mg/mL presented an increase in viability compared to the control group. On the other hand, smaller concentrations presented a similar or lower viability compared to the control group, in general. A new dental material composed of calcium silicate-based cement with 20% zirconium oxide as the radiopacifier showed low cytotoxicity as a promising material to be exploited for root-end filling.
KW - Cell viability
KW - Fibroblasts
KW - MTA
KW - Zirconium oxide
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U2 - 10.1590/0103-6440201300316
DO - 10.1590/0103-6440201300316
M3 - Article
C2 - 26963200
AN - SCOPUS:84954048636
SN - 0103-6440
VL - 26
SP - 587
EP - 591
JO - Brazilian Dental Journal
JF - Brazilian Dental Journal
IS - 6
ER -