TY - JOUR
T1 - Experimental evidences and signal transduction pathways involved in the activation of NF-κB/Rel by angelan in murine macrophages
AU - Jeon, Young J
AU - Kim, Hwan Mook
PY - 2001/6/21
Y1 - 2001/6/21
N2 - In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, activated macrophages to induce the translocation of NF-κB/Rel into nucleus and DNA binding to its cognate site in the promoter of iNOS gene [Immunopharmacology 43 (1999) 1; Immunopharmacology 49 (2000) 275]. In the present study, we showed that angelan induces the transcriptional activation of NF-κB/Rel and investigated the intracellular signal transduction pathways involved in the angelan-induced NF-κB/Rel activation by murine macrophages. Treament of RAW 264.7 cells with angelan resulted in significant activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38, while stress-activated protein kinase/c-Jun NH2 terminal kinase (SAPK/JNK) was not activated by angelan. The specific p38 inhibitor SB203580 abrogated the angelan-induced NF-κB/Rel activation, whereas the selective MAPK/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the NF-κB/Rel induction. Treatment of RAW 264.7 cells with both anti-CD14 Ab and anti-CR3 Ab significantly blocked angelan-induced NF-κB/Rel activation. In conclusion, we demonstrate that angelan induces NF-κB/Rel activation through the CD14 and CR3 membrane receptor and p38 kinase that is critically involved in the signal transduction leading to NF-κB/Rel activation in murine macrophages.
AB - In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, activated macrophages to induce the translocation of NF-κB/Rel into nucleus and DNA binding to its cognate site in the promoter of iNOS gene [Immunopharmacology 43 (1999) 1; Immunopharmacology 49 (2000) 275]. In the present study, we showed that angelan induces the transcriptional activation of NF-κB/Rel and investigated the intracellular signal transduction pathways involved in the angelan-induced NF-κB/Rel activation by murine macrophages. Treament of RAW 264.7 cells with angelan resulted in significant activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38, while stress-activated protein kinase/c-Jun NH2 terminal kinase (SAPK/JNK) was not activated by angelan. The specific p38 inhibitor SB203580 abrogated the angelan-induced NF-κB/Rel activation, whereas the selective MAPK/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the NF-κB/Rel induction. Treatment of RAW 264.7 cells with both anti-CD14 Ab and anti-CR3 Ab significantly blocked angelan-induced NF-κB/Rel activation. In conclusion, we demonstrate that angelan induces NF-κB/Rel activation through the CD14 and CR3 membrane receptor and p38 kinase that is critically involved in the signal transduction leading to NF-κB/Rel activation in murine macrophages.
KW - Angelan
KW - Angelica gigas Nakai
KW - Macrophages
KW - NF-κB/Rel
KW - P38
UR - http://www.scopus.com/inward/record.url?scp=0034995171&partnerID=8YFLogxK
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U2 - 10.1016/S1567-5769(01)00065-0
DO - 10.1016/S1567-5769(01)00065-0
M3 - Article
C2 - 11460313
AN - SCOPUS:0034995171
SN - 1567-5769
VL - 1
SP - 1331
EP - 1339
JO - International Immunopharmacology
JF - International Immunopharmacology
IS - 7
ER -