Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules

Elias M. Puchner; Hermann E. Gaub

doi:10.1002/anie.200905956

Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules

Elias M. Puchner, Hermann E. Gaub

Physics and Astronomy (Twin Cities)

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Figure Presented Feel the force: A new AFM-based singlemolecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.

Original language	English (US)
Pages (from-to)	1147-1150
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	6
DOIs	https://doi.org/10.1002/anie.200905956
State	Published - Feb 1 2010

Keywords

Enzymes
Force spectroscopy
Scanning probe microscopy
Sensors
Single-molecule studies

Access

10.1002/anie.200905956

OpenUrl availability

Full text

Cite this

@article{c327d16fcc754ded82256b1dc47ddfa3,

title = "Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules",

abstract = "Figure Presented Feel the force: A new AFM-based singlemolecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.",

keywords = "Enzymes, Force spectroscopy, Scanning probe microscopy, Sensors, Single-molecule studies",

author = "Puchner, {Elias M.} and Gaub, {Hermann E.}",

year = "2010",

month = feb,

day = "1",

doi = "10.1002/anie.200905956",

language = "English (US)",

volume = "49",

pages = "1147--1150",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "6",

}

TY - JOUR

T1 - Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules

AU - Puchner, Elias M.

AU - Gaub, Hermann E.

PY - 2010/2/1

Y1 - 2010/2/1

N2 - Figure Presented Feel the force: A new AFM-based singlemolecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.

AB - Figure Presented Feel the force: A new AFM-based singlemolecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.

KW - Enzymes

KW - Force spectroscopy

KW - Scanning probe microscopy

KW - Sensors

KW - Single-molecule studies

UR - http://www.scopus.com/inward/record.url?scp=76249114882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76249114882&partnerID=8YFLogxK

U2 - 10.1002/anie.200905956

DO - 10.1002/anie.200905956

M3 - Article

C2 - 20077447

AN - SCOPUS:76249114882

SN - 1433-7851

VL - 49

SP - 1147

EP - 1150

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 6

ER -

Exploring the Conformation-Regulated function of Titin Kinase by mechanical pump and probe experiments with single molecules

Abstract

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this