In situ hybridization histochemistry was used to monitor the expression of cytochrome P450 aldosterone synthase (P450aldo) and cytochrome P450 11βhydroxylase (P45011β mRNA in regenerating rat adrenals. Comparisons were made between regenerating adrenals from unilateral enucleated/unilateral adrenalectomized (ULE/ULA) and bilateral enucleated (BLE) rats. During the first week after enucleation, P45011β mRNA was expressed in all adrenals reflecting the presence of fasciculata cells; however, P450aldo mRNA was detected only in adrenals from ULE/ULA rats suggesting that the glomerulosa cell phenotype was absent after BLE. These findings suggest that the expression of glomerulosa cells during the early period of regeneration is influenced by the presence of a second regenerating adrenal.
Bibliographical noteFunding Information:
This work was supported by the Dept. of Surgery, University of Minnesota, NIH GM50150 and NSF IBN9319097. We thank R. Elde and X. Lin, Dept. of Cell Biology and Neuroanatomy, for help in the development of ISHH. Correspondence to: W. C. Engeland, Ph.D., Dept. of Surgery, Box 809 UMHC, Univ. of Minn. , Minneapolis, MN 55455, (612) 625-4976, Fax: (612) 624-8909