TY - JOUR
T1 - Expression of endogenous muscarinic acetylcholine receptors in Chinese hamster ovary cells
AU - Shou Zhen Wang, Zhen Wang
AU - Sheng Zu Zhu, Zu Zhu
AU - El-Fakahany, Esam E.
PY - 1995/10/15
Y1 - 1995/10/15
N2 - Chinese hamster ovary (CHO) cells are commonly used for expression of the genes of cloned neurotransmitter receptors to study their pharmacology and coupling to signal transduction pathways. It is usually assumed that host cells do not endogenously express the specific receptor under consideration. We demonstrate in this report that CHO cells contain endogenous functional muscarinic acetylcholine receptors which, in some circumstances, might complicate interpretation of data related to the properties of exogenously expressed receptors.
AB - Chinese hamster ovary (CHO) cells are commonly used for expression of the genes of cloned neurotransmitter receptors to study their pharmacology and coupling to signal transduction pathways. It is usually assumed that host cells do not endogenously express the specific receptor under consideration. We demonstrate in this report that CHO cells contain endogenous functional muscarinic acetylcholine receptors which, in some circumstances, might complicate interpretation of data related to the properties of exogenously expressed receptors.
KW - Chinese hamster ovary (CHO) cell
KW - Muscarinic acetylcholine receptor
KW - Nitric oxide (NO)
UR - http://www.scopus.com/inward/record.url?scp=58149326326&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149326326&partnerID=8YFLogxK
M3 - Article
C2 - 8566158
AN - SCOPUS:0028973358
SN - 0922-4106
VL - 291
SP - R1-R2
JO - European Journal of Pharmacology - Molecular Pharmacology Section
JF - European Journal of Pharmacology - Molecular Pharmacology Section
IS - 2
ER -