Abstract
Surrogate light chain (ψLC) genes are transcriptionally active in progenitor B (pro-B) cells before immunoglobulin genes are rearranged. Current hypothetical models suggest that the ψLC proteins may couple with surrogate or conventional heavy chain proteins to form cell surface receptors that signal the progressive differentiation of pro-B, precursor B (pre-B), and immature B cells. Monocional antibodies were produced and used to examine the synthesis, expression, intermolecular interaction, and function of ψLC during B cell differentiation. The results indicate that, while ψLC production spans several developmental stages, cell surface expression is confined to a relatively late stage in normal pre-B cell differentiation, during which receptor cross-linkage does not impede cell growth or B cell differentiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 73-86 |
| Number of pages | 14 |
| Journal | Cell |
| Volume | 73 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 9 1993 |
| Externally published | Yes |
Bibliographical note
Funding Information:These studies have been supported in part by grants Al30679, CA13146, and CA31685 awarded by the US Public Health Service National Institutes of Health. M. D. C. is a Howard Hughes Medical Institute Investigator. We wish to thank Drs. Paul W. Kincade, Stanley J. Korsmeyer, Jack W. Singer, and John F. Kearney for providing cell lines and antibodies; Ms. Julie A. Rehmann for isolation of the IBLIN-1 clone; Dr. G. Larry Gartland for help with the fluorescenceactivated cell sorting analysis; Dr. Peter D. Burrows for helpful criticisms and suggestions: and Mrs. E. Ann Brookshire for preparing the manuscript.